辐照对rhBMP-2骨诱导活性的影响  

Effects of radiation on the osteoinductive activity of recombinant human bone morphogenetic protein 2

作　　者：李幼忱[1] 李宝兴[1] 张育敏[1] 陈学英[1] 康悦[1] 张康蓉[1] 孙世荃[1] 

机构地区：[1]山西省医用组织库,太原030006

出　　处：《中华骨科杂志》2005年第3期176-180,共5页Chinese Journal of Orthopaedics

基　　金：山西省青年科学基金资助项目(20011032)

摘　　要：目的探讨不同剂量γ射线辐照对基因重组人骨形态发生蛋白2(rhBM P-2)骨诱导活性的影响及胶原载体的作用。方法以胶原海绵为载体制成rhBM P-2胶原复合物,分为4类:(Ⅰ)对照组:复合物未辐照;(Ⅱ)复合物辐照组:剂量为15、20、25和50kGy;(Ⅲ)载体辐照组:胶原载体接受50kGy辐照,然后复合未辐照的rhBM P-2;(Ⅳ)BM P辐照组:将rhBM P-2辐照25和50kGy,然后复合于胶原载体。各类BM P胶原复合物植入大鼠股骨肌袋内,术后2周和4周取出包块,进行大体、X线和组织学观察,通过组织形态分析对成骨进行定量分析。结果术后2周,复合物辐照15、20和25kGy组与对照组包块的大体、X线和组织学所见相似,包块内见大量未成熟的编织骨,成骨定量分析见20kGy组与对照组差异无统计学意义;复合物辐照25kGy组新生骨量明显低于复合物辐照15kGy组(P<0.05);复合物辐照50kGy和BM P辐照组无新骨形成。术后4周,各组均出现含有骨髓的成熟骨组织,其中复合物辐照25kGy组与对照组相似;复合物辐照50kGy组有成熟骨但数量较少;载体辐照和BM P辐照组新生骨量明显少于对照组。结论20和25kGy剂量辐照对rhBM P-2骨诱导活性没有影响或仅有一过性抑制;在辐照过程中,胶原载体对BM P具有保护作用。Objective To study the effects of various doses of ionizing radiation on the osteoinductive activity of recombinant human bone morphogenetic protein 2 (rhBMP-2) and the role of collagen carrier. Methods BMP complex was prepared with rhBMP-2 and collagen sponge as a carrier. The osteoinductive activity of rhBMP-2 was evaluated by heterotopic bone formation. According to the conditions of gamma irradiation, the experiments were divided into 4 groups: (Ⅰ) non-irradiated complex as control; (Ⅱ) irradiated complex with 15, 20, 25 or 50 kGy; (Ⅲ) irradiated carrier: collagen sponge was irradiated with 50 kGy and then mixed with non-irradiated rhBMP-2; (Ⅳ) irradiated BMP: only rhBMP-2 was irradiated with 25 or 50 kGy and then mixed with non-irradiated collagen sponge. The BMP complexes were implanted into thigh muscle pouches in 34 Wistar rats, respectively. All collagen mass in muscle pouches were procured two or four weeks after implantation and examined macroscopically, radiographically and histologically. Quantitative analyses of heterotopic bone formation were evaluated by histomorphometry. Results Two weeks after implantation, collagen mass procured from muscle of irradiated complexes with 15, 20, 25 kGy are almost the same with that of control in size, shape and new bone formation, which are composed with richly vascularized connective tissue and immatured woven bone, but only the quantity of new bone in the group of 25 kGy reduced significantly(P< 0.05). There was hardly bone formation in groups of irradiated complex with 50 kGy and irradiated BMP 2 weeks postoperatively. After 4 weeks, however, matured bone with marrow appeared in all groups including 50 kGy group; The new bone of irradiated complex with 25 kGy was similar as that of control; But that of irradiated complex with 50 kGy and irradiated BMP was far less than that of control. The new bone formation of irradiated carrier group was also far less than that of control distinctly. Conclusion The osteoinductive activity of rhBMP-2 is slightly reduc

关 键 词：RHBMP-2 对照组 胶原 骨诱导 辐照 包块 术后 成熟 剂量 活性 

分 类 号：R68[医药卫生—骨科学]