COX-2基因与基质金属蛋白酶在星形细胞瘤中的表达及二者相关性研究  

Expression and Relationship of Cyclooxygenase-2 and Matrix Metalloproteinase in human Astrocytoma

作　　者：吴涛[1] 袁先厚[1] 文志华[1] 马超[1] 吴志敏[1] 

机构地区：[1]武汉大学中南医院神经外科,430071

出　　处：《肿瘤防治研究》2005年第3期135-137,F003,共4页Cancer Research on Prevention and Treatment

摘　　要：目的　探讨COX -2基因与基质金属蛋白酶在星形细胞瘤中的表达及二者相关性。方法　用原位杂交的方法检测星形细胞瘤组织中 COX -2 mRNA的表达;用 Ultrasensitive S P法(链霉素抗生物素蛋白 过氧化物免疫组化染色)检测同等标本中基质金属蛋白酶 2 和基质金属蛋白酶 9 蛋白的表达;并将COX-2 mRNA 与基质金属蛋白酶的表达作相关性分析。结果　 COX- 2 mRNA及 MMP2、MMP9在星形细胞瘤中高度表达,表达阳性率分别为62.69%、56.70%和58.20%;COX- 2 mRNA与 MMP2 和MMP9均有正相线性关系,Pearson积距相关系数分别为0.260、0.347,P<0.05。结论　 COX -2 基因和基质金属蛋白酶在星形细胞瘤组织中的表达密切相关,说明它在星形细胞瘤向周围组织侵袭中起重要作用。Objective Investigate expression and relationship of Cyclooxygenase 2 and matrix metalloproteinase(MMP) in human astrocytoma.Methods In this study, COX 2 mRNA was investigated in 67 astrocytomas and 8 normal brain specimens by using in situ hibridization study. Metalloproteinase 2(MMP2) and metalloproteinase 9(MMP9) expression was investigated in 67 astrocytomas and 8 normal brain specimens by immunohistochemistry.Results Expression of COX 2 mRNA , MMP2 and MMP9 was observed in astrocytoma and not in normal brain specimens. The expression rate of COX 2 mRNA , MMP2 and MMP9 were 62.69 %、 56.70 % and 58.20 %.Conclusion Astrocytoma invasion strongly connects with COX 2 gene. The study suggests that COX 2 gene may perform an important role in human astrocytoma invasion.

关 键 词：环氧化酶-2 星形细胞瘤 基质金属蛋白酶 

分 类 号：R739.41[医药卫生—肿瘤]