应用多重标记法探讨经典型Hodgkin淋巴瘤H/RS细胞的细胞属性  

Primitive Study of Cell Lineage of H/RS Cell in Classical Hodgkin Lymphoma by Using Multiple Staining Techniques

作　　者：郗彦凤[1] 王晋芬[1] 焦士兰[1] 殷卫东[1] 白文启[1] 

机构地区：[1]山西省肿瘤医院病理科,太原030013

出　　处：《肿瘤防治研究》2005年第3期155-157,F003,共4页Cancer Research on Prevention and Treatment

摘　　要：目的　探讨经典型Hodgkin淋巴瘤H/RS细胞的细胞属性。方法　应用免疫组化多重标记法和原位杂交技术检测CD3、CD20、CD15、CD30、CD10、MPO、bcl 6、κmRNA和λmRNA在 62 例经典型Hodgkin淋巴瘤H/RS细胞的表达。结果　62 例的H/RS细胞CD30 全部阳性,41 例表达 CD15,只有5例表达CD20,均不表达 CD3、bcl 6、CD10、κmRNA、λmRNA。结论　经典型 Hodgkin淋巴瘤的 H/RS细胞为生发中心后期分化的B细胞起源;H/RS细胞对B系标记有较大的变异。它不表达免疫球蛋白轻链κ和λ,可能是这些肿瘤细胞无免疫球蛋白的合成。Objective To investigate the lineage of H/RS cell in classical Hodgkin lymphoma. Methods To analysis the expression of CD3、CD20、CD15、CD30、CD10、MPO、bcl 6、κ mRNA and λ mRNA in 62 cases of H/RS cells in classical Hodgkin lymphoma by using immunohistochemical multiple staining technique and in situ hybridizations. Results In all 62 cases ,the H/RS cells were all positive for CD30 ,41 cases positive for CD15, only 5 cases positive for CD20, all cases negative for CD3、MPO、bcl 6、CD10、κ mRNA and λ mRNA. Conclusion H/RS cells of classical Hodgkin lymphoma were originated from later stage germinal center developed B cells. H/RS cells have great varies for B lineage markers . H/RS cells were negative for immunoglobulin κ and λ due to the neoplasm cells can’t synthesize of immunoglobulin. H/RS cells were not antigen driven, there have some unclearly mechanism in its hyperplasia.

关 键 词：经典型Hodgkin淋巴瘤 H/RS细胞 细胞属性 免疫组化多重标记 原位杂交 

分 类 号：R733.4[医药卫生—肿瘤]