猪γ-干扰素基因真核重组表达质粒的构建  

Construction of recombinant eukaryotic expression plasmid of porcine IFN-gamma

作　　者：任慧英[1] 郭鑫[1] 杨汉春[1] 陈艳红[1] 查振林[1] 

机构地区：[1]中国农业大学农业部预防兽医学重点开放实验室,北京海淀100094

出　　处：《中国兽医杂志》2005年第3期5-7,共3页Chinese Journal of Veterinary Medicine

基　　金：国家 8 63计划课题 (2 0 0 2 AA2 413 41) ;国家十五科技攻关计划子课题 (2 0 0 2 BA5 14 A-16-0 5 )

摘　　要：用 RT- PCR方法从长白猪脾脏淋巴细胞中扩增出猪 γ-干扰素 (IFN- γ)基因 ,经克隆测序表明与已发表序列同源性为10 0 %。将目的基因插入表达载体 pc DNA3.1(+) ,构建出真核重组表达载体 IFN-γ- pc DNA。将重组质粒转染 COS7细胞 ,检测转染细胞培养上清 IFN- γ活性 ,结果表明转染上清对猪繁殖与呼吸综合征病毒有抑制作用。IFN-gamma(IFN-γ) gene of Landrace porcine was amplified by RT-PCR, and sequencing result was showed that the cloned gene had 100% homogeneity with the published sequence. The recombinant eukaryotic expression plasmid IFN-γ-pcDNA was constructed by inserting IFN-γ gene into pcDNA3.1(+).The recombinant plasmid was transfected into COS7 and the supernatant showed inhibition activity to porcine reproductive and respiratory syndrome virus (PRRSV).

关 键 词：IFN-γ γ-干扰素 目的基因 转染 真核 重组表达质粒 培养上清 COS7细胞 表达载体 序列同源性 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]