人卵巢癌细胞系克隆化亚系的转移潜能研究  

The Research of Metastatic Potential in Human Ovarian Cancer Cell Subclones

作　　者：陈洁[1] 刘鸣[1] 汤春生[1] 

机构地区：[1]山东大学山东省立医院妇产科

出　　处：《中国肿瘤临床》2005年第6期349-351,共3页Chinese Journal of Clinical Oncology

基　　金：山东省优秀中青年科学家科研奖励基金资助(编号:01BS56)

摘　　要：目的:克隆具有不同转移潜能的癌细胞亚系,并对其侵袭转移能力进行探讨。方法:应用有限稀释法对卵巢癌细胞系SKOV3进行单细胞克隆,共分离出14个克隆亚系,根据细胞电泳率,筛选出3个电泳率差异较大的克隆亚系(S1、S6和S14)。并应用体内外试验包括生长曲线、人工基底膜侵袭试验、裸鼠异种接种及自发转移试验,检测各亚系的生物学特性及其侵袭转移能力。结果:经体内外多项实验检测证明:S1为高转移亚系,体外生长快,侵袭人工基底膜细胞数显著多于其他二者;而S14为不转移亚系,体外生长慢,侵袭人工基底膜细胞数显著少于其他二者。S1裸鼠体内100%成瘤,70.00%转移;而S14裸鼠体内仅20.00%成瘤,无一例发生转移。结论:该系统为来自同一肿瘤母系,遗传背景完全相同,却具有不同转移潜能的异质性克隆,为肿瘤转移机制的研究和克隆肿瘤转移相关基因提供了良好模型。Objective: To isolate and characterize cancer cell subclones with different metastatic potential from human ovarian cancer cell line. Methods: Three sublines with different metastatic potential from human ovarian cancer cell line-SKOV3(S1, S6andS14) were established, using cell clone technique and cell electrophoresis. The invasiveness and migratory capacity among the three subgroups were observed by vitro growth, Matrigel invasion assay and in vivo tumorigenicity and spontaneous metastasis assay in nude mice. Results: Among the three subclones, S1 expressed as the highly metastatic phenotype, with 70.00% metastasis frequency 6 weeks after subcutaneous inoculation into nude mice, whereas S14 was not metastatic. In vitro, S1 was found to be the most highly invasive cell line in Matrigel invasive assay, while S14 was found to have the least invasive ability. Conclusion:Successful establishment of these subclones with different metastatic potential may be valuable for further study on the molecular mechanisms of cancer metastasis and the cloning of related genes of cancer metastasis.

关 键 词：卵巢肿瘤 肿瘤转移 细胞系 克隆 

分 类 号：R737.31[医药卫生—肿瘤]