福建省新分离3株乙型脑炎病毒的分子特征鉴定  

Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province

作　　者：陈端[1] 王环宇[2] 付士红[2] 宋宏[2] 邓娟[2] 杨益良[2] 梁国栋[2] 

机构地区：[1]福建省疾病预防控制中心病毒室,福州350001 [2]中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室

出　　处：《中华实验和临床病毒学杂志》2005年第1期5-8,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助 (3 0 170 0 46)

摘　　要：目的　确定福建省新分离乙型脑炎病毒 (JEV)的基因分型及其E区段氨基酸序列特征。方法　用逆转录聚合酶链反应 (RT PCR)扩增并克隆新分离JEV(0 2 4 1、0 2 4 3、0 2 10 2 )的PrM、E区段核苷酸序列 ,测序后应用ClustalX软件做碱基配对和比较分析 ,种系发生采用PHYLIP软件包分析。结果　新分离的 3株JEV属于基因Ⅲ型 ,E区段核苷酸和氨基酸与减毒活疫苗株SA 14 14 2株的同源性均在 96 %以上 ,在关键结构域有部分氨基酸差异。结论　福建省新分离的 3株病毒属于基因Ⅲ型JEV 。Objective To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene. Methods PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program. Results Newly isolated Japanese encephalitis viruses belonged to genotype Ⅲ, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains. Conclusion Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype Ⅲ. E protein of the isolates showed some differences as compared with vaccine strains.

关 键 词：乙型脑炎病毒 JEV 分离 基因 E蛋白 分子特征 减毒活疫苗 区段 氨基酸 同源性 

分 类 号：R373[医药卫生—病原生物学] R512[医药卫生—基础医学]