人卵巢癌3AO细胞不同转移潜能克隆株的建立  

Establishment of the monoclonal cell sublines with different metastatic potentials from human ovarian cancer cell 3AO

作　　者：韦德英[1] 刘鸣[1] 汤春生[1] 

机构地区：[1]山东大学山东省立医院妇产科,山东济南250021

出　　处：《山东大学学报（医学版）》2005年第3期251-253,256,共4页Journal of Shandong University:Health Sciences

基　　金：山东省优秀中青年科学家科研奖励基金(01BS56)。

摘　　要：目的:建立不同转移潜能的卵巢癌克隆细胞株。方法:通过克隆技术和细胞电泳,从卵巢癌细胞系3AO中筛选出3个电泳率差异较大的克隆亚系(A1、A5和A11)。并通过生长曲线、软琼脂集落形成实验、移动实验、体外侵袭实验,比较它们的生物学特性及其侵袭转移能力。结果:A1的电泳速度最快, 为(15.30±0.67)μm/s,细胞群体倍增时间为20.55 h,软琼脂集落形成数及侵袭人工基底膜的细胞数多于其他两者,为高转移潜能克隆株。A11的电泳速度为(6.20±0.72)μm/s,群体倍增时间为38.48 h,软琼脂集落形成数及侵袭人工基底膜的细胞数少于其他两者,为低转移潜能克隆株。结论:此异质性克隆系统来自同一肿瘤母系,遗传背景相似,却具有不同的转移潜能,为研究肿瘤转移机制和克隆肿瘤转移相关基因提供了良好模型。Objective: To isolate and characterize cancer cell subclones with different metastatic potentials from human ovarian cancer cell line 3AO. Methods: By cell clone technique and cell electrophoresis, three sublines (A1, A5 and All) with different metastatic potentials from 3AO were established. The invasiveness and migratory capacities in the three subgroups were observed by vitro growth, Boyden chamber and soft agar cloning. Results: The electrophoretic mobility of A1, A5 and All was (15.30±0.67) μm/s, (11.02±0.60) μm/s and (6.20±0.72) μm/s respectively. The population doubling time was 20.55 h, 28.99 h and 38.48 h respectively. In vitro, A1 was found to be a high invasive cell line in matrigel invasive assay and formed 45 clones in soft agar cloning assay. On the contrary, A11 was found to have low invasive ability and only formed 13 clones. Conclusion: Successful establishment of these subclones with different metastatic potentials may be valuable for further study on the molecular mechanisms of cancer metastasis and cloning of cancer metastasis related genes.

关 键 词：卵巢肿瘤 肿瘤侵润 肿瘤转移 细胞系 克隆 

分 类 号：R737.31[医药卫生—肿瘤] R7337[医药卫生—临床医学]