姜黄素对人卵巢癌细胞株HO-8910增殖和凋亡的影响  

Effects of curcumin on proliferation and apoptosis of human ovarian cancer cell lineHO-8910 in vitro

作　　者：王清[1] 王淑玉[1] 贾雪梅[1] 黄丽[2] 曾怡[2] 唐桂霞[2] 

机构地区：[1]南京医科大学第一附属医院妇产科,江苏南京210029 [2]南京医科大学微生物学与免疫学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2005年第4期262-265,277,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金项目(NY03029)

摘　　要：目的:探讨姜黄素(curcumin)对体外培养人卵巢癌细胞株HO鄄8910细胞增殖、细胞周期调控及细胞凋亡的影响。方法:选用人卵巢癌细胞株HO鄄8910进行体外培养,以1.25-20μg/ml姜黄素分别处理HO鄄8910细胞6鄄24h,倒置相差显微镜观察细胞形态,MTT法检测细胞增殖,流式细胞仪检测凋亡和细胞周期,SABC免疫组化检测细胞凋亡相关蛋白Fas、Fas鄄L、Bcl鄄2及Bax的表达。结果:姜黄素对HO鄄8910生长有抑制作用,并呈剂量时间依赖性。流式细胞仪分析证实姜黄素能使HO鄄8910细胞积聚在S和G2/M期,并出现亚二倍体凋亡峰。Fas、Fas鄄L、Bcl鄄2表达均上调,Bax加药前后均为阳性。结论:姜黄素对人卵巢癌细胞株HO鄄8910的增殖具有显著的抑制作用;Fas、Fas鄄L、Bcl鄄2的表达上调可能参与了诱导细胞凋亡。Objective:To investigate the regulatory effects of curcumin on proliferation and apoptosis of human ovarian cancer cell line HO-8910 in vitro. Methods:Human ovarian cancer cell line HO-8910,cultured in vitro,was treated with 1.25-20 μg/ml curcumin for 6～24 h. Morphological changes of cells were observed with invert microscope, and growth inhibition rates of HO-8910 cells were measured with MTT method. The cell cycle distribution and apoptosis were detected by flow cytometic analysis. In addition, the expression of Fas,Fas-L,Bcl-2 and Bax in HO-8910 cell was observed by SABC immunohistochemistry. Results: Curcumin inhibited the proliferation of HO-8910 cells in a dose-and time-dependent manner. The cell growth was arrested at S and G2/M stage and aneuploid peak was observed by FCM. The expression of Fas,Fas-L,Bcl-2 was increased while the expression of Bax was both positive before and after treatment. Conclusion:The curcumin not only inhibits proliferation but also induces apoptosis of human ovarian cancer cell line HO-8910.

关 键 词：姜黄素 卵巢癌 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]