维生素E琥珀酸酯对人胃癌细胞增殖分化和热休克蛋白70表达的影响  

Effect of Vitamin E Succinate on Cell Proliferation, Differentiation and Expression of Heat Shock Protein 70 in Human Gastric Carcinoma

作　　者：赵岚[1] 李红卫[1] 吴坤[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,黑龙江哈尔滨150001

出　　处：《癌变．畸变．突变》2005年第2期87-89,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(No.39870662)

摘　　要：背景与目的 :研究维生素E琥珀酸酯(VitaminESucciaate,VES)对人胃癌SGC_7901细胞增殖和分化作用及其可能机制。材料与方法 :采用噻唑蓝(3_[4,5_dimethylthiazol_2_yl]_2,5diphenyltetrazoliumbroide,MTT)法检测细胞的生长 ;流式细胞术检测细胞周期的分布 ;分光光度法检测分化标志酶的活性 ;WesternBlot法检测不同剂量VES对人胃癌SGC_7901细胞热休克蛋白70(HSP70)的影响。 结果 :VES可抑制SGC_7901细胞的生长 ;VES作用48h可使细胞出现G0/G1期停滞 ,分化标志酶AKP、LDH活性降低 ,并使HSP70蛋白表达降低。结论 :VES可抑制SGC_7901细胞的生长并诱导分化 ,其机制可能是通过抑制HSP70的表达而使细胞发生G0/G1 期阻滞 ,从而抑制肿瘤细胞的增殖 ,诱导SGC_7901细胞发生分化。BACKGROUND&AIM: To study the effect of vitamin E succinat(VES)on the human gastric carcinoma SGC-7901cell and the possible mechanism. MATERIAL AND METHODS: Using cell culture in vitro,the effect of VES at the dose of5,10μg/ml were measured by MTT method for cell growth rate,by flow cytometry for cell cycle distribution,by spectrophotometer for enzyme activity,by western blotting for the expression of HSP70(heat shock protein70). RESULTS: After treated with VES,SGC-7901cells were led to growth inhibition,cell cycle arrest,AKP,LDH activity suppressant.Western blotting analysis demonstrated that the expression of HSP70in SGC-7901cells was markedly decreased. CONCLUSION: HSP70is involved in VES induced growth inhibition and differentiation of SGC-7901cells,and associate with cell cycle arrest.

关 键 词：维生素E琥珀酸酯 胃癌 诱导周期 热休克蛋白70 

分 类 号：R735.2[医药卫生—肿瘤]