促性腺激素释放激素类似物预防化疗对大鼠卵巢损伤作用的研究  

Study on Effect of Gonadotropin-releasing Hormone Analogues in Preventing Chemeotherapy-induced Ovarian Damage

作　　者：马晓玲[1] 高云荷[1] 郭红宇[1] 李淑兰[2] 

机构地区：[1]兰州医学院第二附属医院妇产科,兰州730000 [2]甘肃省妇幼保健院病理科,兰州730000

出　　处：《生殖与避孕》2005年第3期137-142,共6页Reproduction and Contraception

摘　　要：目的:探讨促性腺激素释放激素(GnRHa)在减轻化疗所致的大鼠卵巢功能损害的作用。方法:选用2-3月龄Wister雌性大鼠40只,随机分为正常对照组、环磷酰胺(CTX)组、GnRHa组、CTX+GnRHa组。用药后比较4组大鼠卵巢、子宫湿重和形态学变化,并采用放射免疫方法测定4组大鼠FSH、E2。结果:光镜下可见GnRHa能明显改善CTX对卵巢、子宫的损害;CTX组与正常对照组比较FSH升高、E2水平明显降低(P<0.01),子宫和卵巢重量减轻(P<0.01)。CTX使各期卵泡总数显著减少,GnRHa使生长卵泡数、成熟卵泡数减少,而初级卵泡数增加,且总卵泡数增加。CTX+GnRHa组保存了大部分初级卵泡,总卵泡数增加。结论:GnRHa的先期应用对预防化疗所致的大鼠卵巢损伤有一定的保护作用。Objective: To investigate the effect of Alarelin in preventing chemotherapy-induced ovarian failure in rats. Methods: Young female Wistar rats of 2-3 months were randomly devided into four groups: the control group, CTX group, GnRHa(Alarelin) group, CTX+GnRHa group. Radioimmunoassay was used to analyse blood level of follicle-stimulating hormone (FSH) and estradiol (E2) among the four groups. The ovary weight and histomorphological features of ovary and uterus were also observed. Results: As compared with the control group, FSH levels in the CTX group significantly increased, E2 level significantly decreased (P<0.01), and weight of ovary and uterus of CTX group were higher than that of the control group. CTX treatment produced a significant reduc- tion in the total number of follicle. Alarelin significantly reduced the number of medium-large follicles and increased the number of small ones, the total number of follicles increased. Chronic Alarelin treatment resulted in preserving of most small follicles and increased the total number of follicles. Alarelin showed markedly effects on improving the ovarian and uterine morphological construction of rats. Conclusion: It was demonstrated that Alarelin can reduce the chemotherapy-induced ovarian damage in rats.

关 键 词：促性腺激素释放激素类似物(GnRHa) 环磷酰胺(CTX) 卵巢功能 

分 类 号：R73-36[医药卫生—肿瘤]