骨桥蛋白在胃癌中的表达及与胃癌侵袭转移的关系  

Osteopontin expression and its relation to invasion and metastases in gastric cancer

作　　者：张东涛[1] 袁静[2] 杨力[3] 郭新宁[3] 郝志明[4] 韩者艺[4] 吴开春[4] 樊代明[4] 

机构地区：[1]宁夏大学生命科学学院生物重点实验室,银川750021 [2]解放军第五医院妇产科 [3]宁夏医学院附属医院消化内科 [4]第四军医大学西京医院消化病研究所

出　　处：《中华肿瘤杂志》2005年第3期167-169,共3页Chinese Journal of Oncology

基　　金：国家自然科学基金资助项目 (3 0 160 0 3 3 )

摘　　要：目的　探讨骨桥蛋白 (OPN)在胃癌侵袭转移中的作用。方法　采用免疫组织化学SABC法检测OPN、核因子 κBp6 5 (NF κBp6 5 )和基质金属蛋白酶 9(MMP 9)在 12例非癌胃组织和 72例胃癌标本中的表达及分布。结果　OPN、NF κBp6 5和MMP 9的阳性表达率 ,在 12例非癌胃组织中均为 0 ,在 30例无淋巴结转移胃癌组织中分别为 4 3.3%、4 0 .0 %和 4 6 .7% ;在 2 6例有淋巴结转移胃癌组织中分别为 76 .9%、73.1%和 80 .8% ,与无淋巴结转移的胃癌组织的差异有统计学意义 (P值分别为 0 .0 11,0 .0 13和 0 .0 0 9) ;在 16例有淋巴结转移且伴胃外器官转移的胃癌组织中分别为 87.5 %、81.3%和 93.8% ,与无淋巴结转移的胃癌组织差异有统计学意义 (P值分别为 0 .0 0 4 ,0 .0 0 7和0 .0 0 2 )。胃癌组织中 ,OPN与NF κB及NF κBp6 5与MMP 9的阳性表达率有差别 ,但有相关关系 ,r分别为 0 .6 7和 0 .72。结论　OPN与胃癌的侵袭转移有关 ,可能机制是通过活化NF κB ,上调转移相关分子MMP 9的表达 ,从而促进癌细胞的侵袭转移。Objective To investigate the correlation between expression of the osteopontin (OPN) and invasion and metastases in gastric cancer. Methods The expression of OPN, NF κB p65 and matrix metallo proteinase 9 (MMP 9) was detected by immunohistochemistry in non cancer gastric tissue ( n =12 cases) and gastric cancer tissue ( n =72 cases). Results (1) OPN, NF κB p65 and MMP 9 were not expressed in 12 non cancer gastric tissue samples(group A). Their expression rates were 43.3%, 40.0% and 46.7% respectively in 30 gastric cancer samples without lymph nodes metastasis(group B), but they increased to 76.9 %, 73.1% and 80.8% in 26 gastric cancer samples with lymph nodes metastases (group C), and 87.5 %, 81.3% and 93.8% respectively in 16 gastric cancer samples with lymph node and distant metastases(group D). (2) There were statistically significant differences in their expressions between group D and group B( P a =0.004, P c =0.007, P e =0.002) , and between group C and group B ( P b =0.011, P d =0.013, P f = 0.009) .(3) Despite some differences in positive expression rates, correlations existed between OPN and NF κB p65, and between NF κB p65 and MMP 9( P 1 =0.042, P 2 =0.013; r 1 =0.67, r 2 =0.72). Conclusion Osteopondin espression is closely related to the invasion and metastases of gastric cancer. It may upregulate the expression of metastasis related molecule MMP 9 by activating NF κB pathway.

关 键 词：胃癌组织 淋巴结转移 MMP-9 OPN 癌侵袭 表达 NF-κB 结论 可能机制 意义 

分 类 号：R735.2[医药卫生—肿瘤]