缺血－再灌注兔心肌肌浆网自由基的测定  

^Direct determination of free radical generation in sarcoplasmic reticulum in isolated reperfused rabbit heart

作　　者：王明勇[1] 李香善[1] 李丹[1] 金基焕[1] 郑莹光[1] 王萍[1] 董凤霞[1] 

机构地区：[1]延边医学院附属医院胸心外科,延边医学院心血管研究室,吉林大学测试中心

出　　处：《延边医学院学报》1994年第4期242-246,共5页Journal of Medical Science Yanbian University

基　　金：国家自然科学基金

摘　　要：应用电子自旋共振波谱仪（ＥＳＲ）直接检测了缺血－再灌注兔心肌肌浆网自由基的变化，以探讨肌质网系统与氧自由基的关系。实验中将２０只兔随机分为再灌注对照组、超氧化物歧化酶（ＳＯＤ）组、ＡＴＰ－氯化镁组和人参皂甙Ｒｅ组。实验结果，ｇ值２．００４６处为半醌自由基波谱，其相对浓度各组依次为７８．９４±２．１２６，１４．４６±２．８６，２０．６５±７．６５，１４．６６±３．６７（ｘ±ＳＤ），对照组与用药组均有显著性差异（Ｐ＜０．０５），表明缺血－再灌注兔心肌肌浆网产生大量的自由基，用ＥＳＲ可以直接检测到半醌自由基，外源性高能磷酸盐制剂ＡＴＰ－氯化镁及人参皂甙Ｒｅ与超氧化物歧化酶一样，发挥清除兔心肌肌浆网自由基的作用。Oxygen free radicals of sarcoplasmic reticulum of ischemia reperfused myocardium in rabbit were directly detected by electron spin resonance(ESR) to study the relation between the sarcoplasmic reticulum system and oxygen free radicals .20 rabbits were randomly divided into reperfusion, superoxide dismutase, ATP-MgCl_2 and ginsenoside Re groups. The results showed that g was 2. 004 6 that means the spectrum of semiquinal free radical and the relative strength of ESR signal in per gram of membrane protein of the four groups was 78. 94±2. 126, 14. 46±2. 86, 20. 65±7. 65 and 14. 66±3. 67(x±SD) ,respectively. The strength of the latter three groups was significantly different comparing with that of reperfusion group(P<0.05). The results implicated that there is an increase of the free radicals in the sarcoplasmic reticulum of reperfused myocardium in rabbit, moreover, ATPMgCl_2 and ginsenoside Re play an apparent role in scavenging the free radicals, both could be used as free radical scavengers.

关 键 词：氧自由基 肌浆网 心肌 缺血再灌注 电子自旋共振 

分 类 号：R363.27[医药卫生—病理学]