河南省艾滋病毒I型膜蛋白基因序列特征和亚型研究  

Study on subtype and sequence of env gene among HIV-1 strain in Henan province

作　　者：崔为国[1] 邢辉[2] 王哲[1] 黄海龙[2] 李宏[1] 薛晓玲[1] 朱新朋[1] 邵一鸣 

机构地区：[1]河南省疾病预防控制中心,河南郑州450003 [2]中国疾病预防控制中心,中国北京100050

出　　处：《河南预防医学杂志》2005年第1期3-5,共3页Henan Journal of Preventive Medicine

摘　　要：目的　了解在河南省流行的人类免疫缺陷病毒(HIV)毒株的流行情况及亚型特征。方法　用套式聚合酶反应(nested -PCR)对28份河南省内的HIV-1感染者外周血单个核细胞(PBMc)中的膜蛋白基因进行扩增,并对C2-V3及其邻区350 -450个核苷序列进行测定和分析。结果　28份样品间的基因离散率为5.72%,与流行于云南的(泰国B)毒株基因离散率 为5.80%,与其它国际亚型,欧美B、A、C、D、F、G、H、J、O亚型基因离散率均在18.05%以上。系统树分析显示,28份毒株与 泰国B亚型聚在一起而远离其它国际亚型。其毒株V3环四肽序列泰国B亚型基因型GPGQ8例占28.57%,欧美B亚型基 因型GPGR13例占46.43%.结论　河南省流行的HIV株属B′亚型,其毒株来自与泰国接壤的云南,在河南流行的时间约在 6～10年,其V3环顶端四肽序列特征以GPGR为主。Objective To investigate the prevalent status and characteristic of subtypes of HIV-1 in Henan province . Methods HIV-1 env gene was amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from 28 HIV-1 seropositive infectors of Henan in 2002.The C2-V3 region (about 450bp)of them was sequenced . Results The innergroup gene divergence of 28 sample is 5.72%,and 5.80% with reference strains of yunnan origin (Thailand B). The sample gene divergence with other subtypes,France B,A,C,D,F,G,H,J,O were over 18.05%. Phylogenetic tree analysis showed 28 Henan B strains clustered closely to Thailand B and far from other international subtypes. The analysis of V3 loop amino sequence of 28 Henan B strains reveals that V3-tip motif of 8 samples (28.57%)is GPGQ of Thailand B subtype gene ,13 samples (46.43%)GPGR of Europe and America B subtype gene .Conclusion There finding suggest the epidemic of subtype B of HIV-1 in Henan province and it is closely related to those identified in yunnan province . It had existed for about 6-10 year .V3 tip motifs of the HIV-1 isolates were mainly GPGR .

关 键 词：人类免疫缺陷病毒Ⅰ型 亚型 序列测定 基因变异 

分 类 号：R512.9[医药卫生—内科学]