维生素E琥珀酸酯诱导人胃癌细胞分化的作用  

Vitamin E succinate induced differentiation in human gastric carcinoma cell line SGC-7901

作　　者：赵岚[1] 吴坤[1] 李红卫[1] 苑林红[1] 张晓华[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品教研室,哈尔滨150001

出　　处：《中国公共卫生》2004年第11期1308-1309,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助 (39970 4 7)

摘　　要：目的　研究维生素E琥珀酸酯 (VES)对胃癌细胞的诱导分化作用。方法　 5 ,10 μg/ml维生素E琥珀酸酯处理人胃癌SGE - 790 1细胞 ,分别采用噻唑蓝 (MTT)法检测细胞的生长 ;3 H -TdR掺入法测定DNA合成速率 ;流式细胞术检测细胞周期的分布 ;分光光度法检测分化标志酶的活性。结果　VES可使细胞形态学改变 ;抑制SGC -790 1细胞的生长 ,降低DNA的合成速率 ;使细胞出现G0 /G1期停滞 ;分化标志酶碱性磷酸酶 (ALP)、乳酸脱氢酶(LDH)活性降低。结论　VES可诱导SGC - 790Objective To study differentiation-induction effect of vitamin E succinate(VES) on human gastric carcinoma cell line SGC-7901.Methods At the base of cell culture in vitro ,the effect of differentiation-inducing activity of VES at the dose of 5,10?μg/ml were measured by MTT method for cell growth rate;by 3H-TdR incorporation method for DNA synthetic speed;by flow cytometry for cell cycle distribution,by spectrophotometer for enzyme activity.Results After treated with VES,SGC-7901 cells were led to morphologic change,growth inhibition,DNA synthetic speed decrease,cell cycle arrest,and ALP,LDH activity suppressant.Conclusion VES can induce SGC-7901 human gastric cancer cells to undergo differentiation.

关 键 词：维生素E琥珀酸酯(VES) 胃癌细胞 诱导分化 

分 类 号：R361.3[医药卫生—病理学]