肝细胞癌DNA干系倍体分析及其临床意义  

Analysis of Hepatocellular Carcinoma DNAStemline Ploidy and Its Clinical Significance

作　　者：李朋军[1] 夏潮涌[1] 潘运龙[2] 黄中新[1] 覃莉[1] 唐海兰[1] 

机构地区：[1]暨南大学医学院组织胚胎学教研室,广州510632 [2]暨南大学第一医院普外科,广州510632

出　　处：《中国体视学与图像分析》2004年第4期199-202,共4页Chinese Journal of Stereology and Image Analysis

基　　金：广东省自然科学基金项目 (No .0 10 36 7)

摘　　要：目的:测量与分析肝细胞癌DNA干系倍体及其临床意义。方法:使用TIGER细胞图像分析仪测量 4 5例肝细胞癌组织 4 μm、10 μm切片上DNA干系倍体值。4 μm组织切片测量肝细胞癌细胞核DNA的光密度,10 μm组织切片测量单个完整肝细胞癌细胞核的体积,经TIGER细胞图像分析仪计算获得以单个完整肝细胞癌细胞核体积为单位的DNA总量(以体积积分光密度表述),以同一切片内正常淋巴细胞作为内对照,计算其DNA干系倍体值。结果:⑴无 1例DNA干系倍体为二倍体;DNA干系倍体值在 2～ 5范围者 11例;在 5～ 8范围者 2 8例;大于 8者 6例。⑵DNA干系倍体与瘤体大小、有无淋巴结转移、组织学分级、术后生存率等有相关性。结论:DNA干系倍体能较准确反映肝细胞癌的生物学特性,为认识肝细胞癌的病理学特征以及判断预后提供了有价值的客观依据。Objectives: To measure and analysis on hepatocellular carcinoma DNA stemline ploidy and its clinical significance. Methods: DNA stemline ploidy of forty-five hepatocellular carcinoma tissue of 4μm、10μm continuous section were measured using TIGER image analyzer. DNA optical density parameters on 4μm section and volume of single intact cell nuclei on 10μm section were measured, the DNA content of each sample at a unit of volume of single intact cell nuclei, was computed by TIGER image analyzer. The normal lymphocyte nuclei in the same section were used as intra-control for that case, so that the DNA stemline ploidy of each sample in the same case can be valued by TIGER image analyzer. Results: (1) None of the DNA stemline ploidy was diploidy, eleven cases were range from 2 to 5, twenty-eight cases were range from 5 to 8, six cases were over 8. (2) DNA stemline ploidy is correlated with tumor size, lymph node metastasis, histological grading and survival rate after operation. Conclusion: The biological characteristics of hepatocellular carcinoma could be reflected by DNA stemline ploidy accurately, which has objective evidence for realizing the pathologic characteristics and judging the prognosis of hepatocellular carcinoma.

关 键 词：肝细胞癌 DNA干系倍体 细胞图像分析仪 

分 类 号：R735.7[医药卫生—肿瘤]