反相高效液相色谱－紫外吸收法测定灵芝中氨基酸的含量  

The Separation of Amino Acids in Glossy Ganoderma on Reversed Phase Column and Quantitative Determination wiht UV Absorption Detector

作　　者：商振华[1] 于亿年[1] 郭为[1] 蒋辉 王俊德[1] 

机构地区：[1]中国科学院大连化学物理研究所

出　　处：《药物分析杂志》1994年第4期30-33,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金

摘　　要：采用本实验室自行制备的反相高效液相色谱柱，以醋酸钠缓冲液和乙腈－水线性梯度洗脱，在室温下对常见的１６种１－氟－２，　４－二硝基苯（ＦＤＮＢ）衍生化氨基酸进行了有效的分离，用紫外吸收检测器（３６０ｎｍ）和外标定量法对灵芝蛋白中各种氨基酸的含量进行了测定。结果表明：ＦＤＮＢ衍生化氨基酸在一周内是稳定的，回收率在９６％～１０２％之间，相对标准偏差小于４％，灵芝中含有人体必需的赖氨酸、苯丙氨酸和组氨酸等组分。The primary and secondary amino acids produced in precolumn derivatization with 1-fluoro-2.4-dinitrobenzene(FDNB) have been efficiently separated on the reversed phase column prepared in our laboratory at ambient temperature. A simple linear gradient elution with acetate buffer and acetonitrile solution is used for mobile phase during the separation. The method has been successfully applied to the quantitative determination of amino acids of hydrolysed protein of glossy ganoderma. The FDNB derivatives of amino acids are more stable than those in traditional methods. The method has high recovery and good accuracy.

关 键 词：氨基酸 反相柱 紫外吸收检测 灵芝 

分 类 号：R284.1[医药卫生—中药学]