苯骈二氮卓类药物的裂解机制及热裂解薄层色谱测定的研究  

Study on the Pyrolytic Mechanism and Determination of Benzodiazepine Drugs by Pyrolytic-TLC

作　　者：杨悦武 柳正良[1] 孔庆洪 沈敏[1] 王勇[1] 

机构地区：[1]第二军医大学药学院药物分析教研室,上海司法鉴定所

出　　处：《药物分析杂志》1994年第6期29-32,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：本文对苯骈二氮卓类药物的热裂解机制以及其簿层展开后重氮偶合显色的色谱方法测定该类药物及其它含氮药物进行了详细研究。以氯氮　为例，利用ＴＬＣ和ＧＣ－ＭＳ法，证实了其主要裂解产物之一为２－氨基－５－氯苯基苯甲酮，以及色谱显色机制为热裂解产物的重氮偶合显色。建立了用于苯骈二氮类及其它含氮药物分析的裂解薄层色谱法。展开系统Ｓ_１：氯仿－乙酸乙酯－正丁醇－浓氨水（７∶３∶０．９∶０．０５）Ｓ_２：四氯化碳－乙醚－正丁醇－浓氨水（７∶３∶ｌ．５∶０．０３）。临床毒物分析实样测定结果满意。本法简便、快速、灵敏、专属，最低检测限达０．１μｇ，适合其它含芳伯胺类药物分析。Study on the pyrolytic mechanism of benzodiazepines and the determination of benzodiazepines and other nitrogen containing drugs by pyrolytic-TLC is discussed in detail in the article. Chlorodiazepoxide was used as an example, it has been proved that one of the main pyrolytic products is 2-amino-5-chlorobenzophenone by TLC and GC-MS. and the colouring mechanism is that the pyrolytic products on the plate are diazotized with NaNO_2plus concentrate HCl and then coupled with naphthylethylenediamine.At the same time.a pyrolytic-TLC method has been established and applied to the analysis of benzodiazepines and other nitrogen containing drugs, the developing systems are:S_1:chloroform-ethyl acetate-n-butanol-concentrate ammonia (7∶3∶0.9∶0.05),S_2:carbon tetrachloride-ether-n-butanol-concentrate ammonia (7∶3∶1. 5∶0.03). Results from the clinical toxic analysis show that the method is simple, rapid,sensitive and specific,it also has better application. suitable for the analysis of other aromatic primary amine drugs.

关 键 词：苯骈二氮Zuo 裂解薄层色谱 GC-MS法 

分 类 号：R914.1[医药卫生—药物化学]