肺癌细胞核三维结构的体视学定量研究(Ⅰ)  

Quantitative Study on Three-Dimensional Nuclear Structure of The Lung Carcinomas by Stereology Method ( )

作　　者：张晓东[1] 申洪[1] 

机构地区：[1]第一军医大学病理学教研室,广州510515

出　　处：《中国体视学与图像分析》2004年第1期14-20,共7页Chinese Journal of Stereology and Image Analysis

基　　金：国家自然科学基金项目(NO.30271462);广东省科技攻关项目(NO.2KM04501S)

摘　　要：目的:用体视学方法定量揭示肺癌细胞核的三维形态结构特点及变化规律。 方法 收集肺癌手术切除标本腺癌30例、鳞癌20例、腺鳞癌8例、未分化小细胞癌6例、未分化大细胞癌6例及肺癌患者自身正常肺组织8例。每例随机切3个蜡块,HE染色,每张切片随机取3-4个视野,用Image-Pro图像分析软件测试核及细胞,按体视学公式计算以细胞为参照空间时肺癌细胞核的体积密度(Vvn)、表面积密度(Svn)、表面积与体积比(Rsv,n)、数密度(Nvn)、核浆比(Rnp)、平均体积(vn)、平均表面积(sn)、平均自由程(n)及以核为参照系时核仁的数密度(Nvn2)。 结果:Vvn、Svn、Rsv,n、Nvn、vn、sn、Rnp、n、Nvn2均值分别为0.3304、0.2628m-1、0.7838m-1、0.0013m-3、383.7758m3、272.5568m2、0.9242、14.4891m、0.0011m-3,分别是正常肺泡上皮细胞核的2.22倍、1.44倍、61.17%、75.42%、4.41倍、2.56倍、5.22倍、73.95%,其范围分别为0.1432～0.9196、0.0883m-1～1.0411m-1、0.5687m-1～1.1321m-1、0.0002m-3 ～0.0103m-3、89.5911m3～765.0668m3、101.4292m2 ～491.3904m2、0.1672～11.4342、0.3090m～38.8223m、0.0000m-3～0.0059m-3,变异系数分别为0.5257、0.7386、0.1254、1.4079、0.4033、0.2856、2.1030、0.5338、1.0728。 结论: 1.Objective: To reveal quantitatively the characteristics and changing rules of the three-dimensional nuclear structure of the lung carcinomas by stereology. Methods: The mass specimens were obtained from the surgical resections of the lung carcinomas, which were consisted of 30 adenocarcinomas, 20 squamous cell carcinomas, 8 adenosquamous carcinomas, 6 small cell lung carcinomas, 6 large cell lung carcinomas and 8 normal lung mucosas. Three or four views were selected stochastically from each stain which was cut from three paraffin masses selected randomly and stained by Hematoxylin-Eosin. We measured the nucleus and reference space using the Image-Pro analytical software. The parameters of Vvn, Svn, Rsv, n, Nvn, vn, sn, Rnp, n and Nvn2 (numerical density of the nucleolus) were acquired according to the stereologic formulas and compared to analysis the differences among the different groups. The reference space of nucleus was according to the cancer cells and that of nuclei was according to the nucleus. Results: The mean of Vvn, Svn, Rsv,n,Nvn,vn,sn,Rnp,n and Nvn2 was respectively 0.3304, 0.2628靘-1, 0.7838靘-1, 0.0013靘-3, 383.7758靘3, 272.5568靘2, 0.9242, 14.4891靘 and 0.0011靘-3, which was respectively 2.22 times, 1.44times, 61.17 percent, 75.42 percent, 4.41 times, 2.56 times, 5.22 times and 73.95 percent of that of the nuclei of normal alveolar epithelium cell. Their ranges were respectively 0.1432～0.9196, 0.0883靘-1～1.0411靘-1, 0.5687靘-1～1.1321靘-1, 0.0002靘-3 ～0.0103靘-3, 89.5911靘3～765.0668靘3, 101.4292靘2 ～491.3904靘2, 0.1672～11.4342, 0.3090靘～38.8223靘 and 0.0000靘-3～0.0059靘-3. And their coefficients of variability (CV) were 0.5257, 0.7386, 0.1254, 1.4079, 0.4033, 0.2856, 2.1030, 0.5338 and 1.0728, respectively. Conclusions: The quantitative characteristics of the three-dimensional nuclear structure of the small cell lung carcinomas look greatly dissimilar while that of the different histologic sub-types of the non-small cell lung carcinomas look relatively alike

关 键 词：肺癌 体视学 定量 三维结构 

分 类 号：R734.2[医药卫生—肿瘤]