阿曲库铵苯磺酸盐的高效液相色谱分析  

HICH-PERFORMANCE LIQUID CHROMATOGRAPHY OF ATRACURIUM BESILATE

作　　者：刘春胜[1] 王云萍[1] 周同惠[1] 

机构地区：[1]中国医学科学院药物研究所

出　　处：《药学学报》1994年第1期68-73,共6页Acta Pharmaceutica Sinica

摘　　要：反相氰基柱高效液相色谱分析阿曲库铵苯磺酸盐（卡肌宁）样品，采用乙腈－水－磷酸－硫酸四元流动相基线分离并同时测定阿曲库铵苯磺酸盐及相关化合物，甲醇－硫酸流动相系统分离测定阿曲库铵苯磺酸盐的顺－顺、顺－反和反－反三种立体异构体，考察了流动相各组分浓度变化对化合物色谱保留值和分离度的影响。方法重现性好，线性范围较宽，检测限为１０ｎｇ。The separation and determination of atracurium besilate and its three stereoisomerswere studied, Atracurium besilate and its related compounds ,and the three stereoisomers of.(±)atracurium besilate were fairly well separated with a nitrile- bonded phase column By acetonitrile-water-phosphoric acid-sulphuric acid(92 :8:0.4:0.03)mobile phase and methanol-sulphuric acid(1000 :1)mobile phase , respectively. The influence of the component concentrations of the mobilephase system on the retention and resolution was investigated. The results indicate that the methodgives good reproducibility and wide response linearity. The detection limit of atracurium besilate was10 ng.This method can be applied to the routine analysis and clinical determination of atracuriumbesilate。

关 键 词：立体异构体 高效液相色谱 卡肌宁 

分 类 号：R914.1[医药卫生—药物化学]