兼性厌氧纤维素降解菌的筛选和产酶研究  

Screening for combined-anaerobic hypercellulolytic fungi and its cellulolytic enzymes production

作　　者：蔡兴旺[1] 路福平[1] 杜连祥[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《工业微生物》2005年第1期6-9,共4页Industrial Microbiology

摘　　要：从我国天津地区堆肥中分离筛选出一株兼性厌氧的纤维素分解细菌D1,初步鉴定为纤维单孢菌(Cellulomonassp.)。产酶最适碳源为葡萄糖,氮源为复合蛋白胨,酶的最适作用温度和pH值是50℃和6.4,在70℃以下和pH在5.2～8.4里稳定。Cellulomonas sp.D1 producing cellulase(CMCase) was screened from the muck in Tianjin. Higher ~production of CMCase depended on the presence of glucose and polypeptone. The optimal pH and temperature for enzyme ~activity was 6.4 and 50℃,respectively. The pH and temperature for enzyme was stabe within the range of 5.2～8.4 and ~below 70℃,respectively.

关 键 词：氮源 天津地区 蛋白胨 分离筛选 堆肥 纤维素降解 碳源 厌氧 pH值 分解 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]