二十二碳六烯酸对人树突状细胞基因表达谱的影响  

Effect of docosahexaenoic acid on gene expression profiles of human dendritic cells

作　　者：汪灏[1] 李秋荣[1] 马健[1] 李幼生[1] 李宁[1] 黎介寿[1] 

机构地区：[1]南京军区南京总医院解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2005年第2期65-68,共4页Parenteral & Enteral Nutrition

基　　金：国家重点基础研究发展规划项目资助(批准号: 2003CB515502);国家自然科学基金资助(批准号: 30400353)

摘　　要：目的:研究二十二碳六烯酸(DHA)对人树突状细胞 (DCs)成熟过程中基因表达谱的影响。　方法:采用GM CSF及IL 4诱导人外周血单核细胞获得非成熟DCs,经DHA( 50μmol/L)孵育 24h后,再予以LPS(100ng/ml)作用 48h,用cDNA芯片检测人DCs基因表达谱。用RT PCR法验证相关差异表达基因的结果。分别将未经脂肪酸处理的DCs和硬脂酸(SA)处理的DCs作为对照。　结果:经DHA预处理后,成熟的DCs与未经脂肪酸处理的成熟DCs相比,共有 20条基因差异表达 ,约占芯片中基因总数的 29. 4%。其中 18条基因表达下降, 2条基因表达增加。在差异表达的基因中,涉及细胞因子、趋化因子 (受体)及其抗原呈递相关分子基因等。而采用饱和脂肪酸(SA)处理的DCs的表达谱未见明显变化。　结论: 50μmol/LDHA对DCs成熟过程中的基因表达具有显著的影响 ,涉及到细胞因子、趋化因子(受体)及其抗原呈递相关分子基因的调节。Objective: To investigate effect of docosahexaenoic acid (DHA) on gene expression profiles of human dendritic cells. Methods: Dendritic cells were generated from human peripheral blood monocytes in the presence of GM-CSF and IL-4, and then treated with DHA (50 μmol/L) for 24h. Lipopolysaccharide(100 ng/ml) was used for maturation of dendritic cells. Gene expression of different dendritic cells was analyzed by cDNA microarray technology . Results of gene array were verified by RT-PCR. DCs treated with stearic acid(SA) were served as control groups. Results: A total of 20 genes exhibited differential expression in DHA-treated dendritic cells compared with control. Eighteen genes were up-regulated and two genes were down-regulated,which included cytokines, chemokines, chemokine receptors and antigen presentation molecules. Conclusions: DHA has significant suppressive effects on dendritic cells in their maturation procession and the changed pattern is related to cytokines, chemokines, chemokines receptors and antigen presentation molecules.

关 键 词：DC 人树突状细胞 基因表达谱 抗原呈递 趋化因子 细胞因子 受体 DHA 二十二碳六烯酸 成熟过程 

分 类 号：Q987[生物学—遗传学]