含多抗原表位的嵌合SARS-CoV基因疫苗的构建和免疫原性研究  

Construction and Immunogenecity of a Multi-epitope Based SARS-CoV Gene Vaccine

作　　者：汪晓华[1] 童德妍[1] 徐焕宾[1] 熊思东[1] 

机构地区：[1]复旦大学上海医学院免疫学系-教育部分子医学重点实验室-上海基因免疫与疫苗研究中心,上海200032

出　　处：《复旦学报（医学版）》2005年第2期130-133,共4页Fudan University Journal of Medical Sciences

基　　金：上海市科委重点项目 (0 3DZ1910 5 );教育部科技攻关项目

摘　　要：目的　研制基于表位的SARS- CoV基因疫苗 ,为传统灭活或减毒疫苗提供有益的配伍选择。方法　通过网络数据库结合生物软件分析的方法 ,确定可能在SARS- CoV感染过程中起重要作用并具较好抗原性参数的结构区域作为候选抗原表位 ,以密码子优化的方法提高表位串联蛋白的真核表达效率 ,构建了含多抗原表位的嵌合SARS- CoV基因疫苗。结果　多表位串联基因接入真核表达载体后 ,可在真核细胞内高效表达。多表位嵌合SARS -CoV基因疫苗免疫小鼠后 ,可诱导融合蛋白特异的体液免疫反应。结论　该基于多抗原表位的嵌合SARS- CoV基因疫苗可以诱导抗体应答 ,为该疫苗的深入研究奠定了基础。Purpose To develop a multi-epitope based gene vaccine for SARS-CoV. Methods Geno-mic data were analyzed and four epitopes were chosen for vaccine development.The gene encoding those tandem-arranged epitopes were then optimized for eukaryocytic expression.A multi-epitope based gene fragment with the predicted epitopes was synthesized and cloned into relevant vectors. Results After transfection,the gene fragment was proved to be able to express in eukaryocyte in vitro and elicited specific anti-fusion protein antibody in vivo. Conclusions These results suggested that our multi-epitope based gene vaccine is a good candidate for further vaccine development.

关 键 词：SARS-COV 多抗原表位 基因疫苗 免疫原性研究 嵌合 真核表达载体 体液免疫反应 网络数据库 配伍选择 减毒疫苗 软件分析 感染过程 表达效率 高效表达 免疫小鼠 融合蛋白 抗体应答 结构区 抗原性 密码子 细胞内 串联 

分 类 号：R373.1[医药卫生—病原生物学] R392.7[医药卫生—基础医学]