重症急性胰腺炎肿瘤坏死因子-α与低钙血症的关系  

Relationship between hypocalcemia and tumor necrosis factor α in severe acute pancreatitis rats

作　　者：陆逢春[1] 黄鹤光[1] 

机构地区：[1]福建医科大学附属协和医院普外科,福州350001

出　　处：《中华实验外科杂志》2005年第4期397-398,共2页Chinese Journal of Experimental Surgery

基　　金：福建省教育厅科研基金资助项目 (JA0 30 99)

摘　　要：目的　探讨重症急性胰腺炎(SAP)大鼠模型肿瘤坏死因子(TNF) α与血清钙水平的关系。方法　胰管内逆行注射5 %牛磺胆酸钠的方法建立SAP大鼠模型。将SD大鼠分为假手术组2 1只,SAP组2 1只,治疗组2 1只。治疗组为模型制作前15min自阴茎背静脉注入抗肿瘤坏死因子α单克隆抗体(TNF αMcAb ,5mg/kg体重) ,观察血中TNF α与血钙的水平。酶联免疫吸附法(ELISA)测定细胞因子水平。结果　重症急性胰腺炎TNF α水平与血钙之间呈显著负相关(r =-0 .69,P <0 .0 1) ,在发病初期两者关系更紧密(6h组:r =-0 .73 ,P <0 .0 1)。TNF αMcAb能改善胰腺病理变化、降低淀粉酶、降低血清TNF α、升高血钙,6hSAP组血清钙(1.99±0 .0 3 )mmol/L ,TNF αMcAb治疗组血清钙(2 .0 6±0 .0 6)mmol/L ,P <0 .0 5 ;12hSAP组血清钙(1.88±0 .0 5 )mmol/L ,TNF αMcAb治疗组血清钙(1.94±0 .0 5 )mmol/L(P <0 .0 5 )。结论　TNF α可能在SAP并发低钙血症的机制中发挥作用。Objective To investigate the correlation between serum tumor necrosis factor α (TNF-α) and calcium level in the severe acute pancreatitis (SAP).Methods SAP animal model was developed in adult male rats by retrograde injection of 5% sodium taurocholate.Sixty-three male Sprague-Dawley rats were divided into sham operation group ( n =21),SAP group ( n =21),SAP therapy group ( n =21).In SAP therapy group,TNF-αMcAb (5 mg/kg) was infused intravenously 15 min before acute pancreatitis was induced.The levels of serum TNF-α and calcium were tested in different time points.TNF-α was determined by ELISA.Results The level of TNF-α had a close relation to that of serum calcium in the SAP ( r = -0.69, P < 0.01), especially at the initial stage (6 h, r = -0.73, P < 0.01). Pretreatment with TNFα McAb could alleviate the pancreatic pathological change,decrease serum amylase and TNF-α.The serum calcium level in the groups of TNF-α McAb pretreatment was 2.06± 0.06 and 1.94± 0.05 mmol/L respectively 6 and 12 h after SAP onset,significantly higher than that in the groups of SAP ( P < 0.05). Conclusion TNF-α may play a role in the mechanism of hypocalcemia with SAP,especially at the initial stage of SAP.

关 键 词：重症急性胰腺炎 肿瘤坏死因子-Α 低钙血症 抗肿瘤坏死因子Α mol/L (TNF)-Α 酶联免疫吸附法 血清TNF-α McAb 细胞因子水平 大鼠模型 牛磺胆酸钠 单克隆抗体 治疗组 血清钙 SAP 方法建立 SD大鼠 静脉注入 模型制作 发病初期 病理变化 0.05 发挥作用 疾病发 

分 类 号：R576[医药卫生—消化系统]