白细胞介素-1α、6对胰腺癌细胞分泌血管内皮生长因子A、C的调节作用  

Interleukin-1α,6 regulate secretion of vascular endothelial growth factor A,C in pancreatic cancer

作　　者：唐瑞峰[1] 王曙霞[2] 张风瑞[1] 彭利[1] 王顺祥[1] 肖燕[1] 张萌[1] 

机构地区：[1]河北医科大学第四医院肝胆外科,石家庄050011 [2]河北医科大学第四医院皮肤科,石家庄050011

出　　处：《中华实验外科杂志》2005年第4期401-403,共3页Chinese Journal of Experimental Surgery

基　　金：教育部留学回国人员科研启动基金资助项目 (教外司留[2 0 0 2 ] 2 4 7号 )

摘　　要：目的　探讨细胞活素白细胞介素 1α、6(IL 1α、IL 6)对胰腺癌细胞产生和分泌血管内皮生长因子(VEGF)A、C的调节。方法　用Northern杂交和Western杂交法分别分析人胰腺癌细胞株(ASPC 1、CAPAN 1、MIA PaCa 2、PANC 1、COLO 3 5 7和T3M 4)中VEGF A、C基因和蛋白的表达;以细胞活素IL 1α(10 μg/L)或IL 6(10 0 μg/L)刺激细胞株(CAPAN 1和COLO 3 5 7)后用逆转录 聚合酶链式反应技术(RT PCR)分析其VEGF A、C基因的表达。结果　Northern杂交法显示6种胰腺癌细胞株均有4.1kbVEGF A基因和2 .4kbVEGF C基因的表达;Western杂交法显示这6种胰腺癌细胞株均有分子量为43kDa的VEGF A蛋白质和分子量为5 5kDa的VEGF C蛋白质的表达。RT PCR分析法显示IL 1α使细胞株COLO 3 5 7产生VEGF A、C基因分别增加1～2倍、1倍,但对细胞株CAPAN 1无明显刺激作用;而IL 6刺激细胞株CAPAN 1产生VEGF A、C基因分别增加2～5倍、1倍,但对细胞株COLO 3 5 7无明显刺激作用。结论　胰腺癌中血管内皮生长因子A、C和细胞活素IL 1α、IL 6间的相互作用导致了癌细胞的血行和淋巴转移。Objective To explore cytokines interleukin-1α,6 (IL-1α,IL-6) regulated the expression of vascular endothelial growthfactor A,C (VEGF-A,-C) in cultured human pancreatic cancer cell lines.Methods By using Northern blot and Western blot methods,the expression of gene and protein of VEGF-A,-C in all 6 tested cell lines (ASPC-1,CAPAN-1,MIA-PaCa-2,PANC-1,COLO-357 and T3M4) was detected respectively.By using reverse-transcription polymerase chain reaction (RT-PCR) methods,the VEGF-A,-C expression was detected in cultured human pancreatic cancer cell lines(CAPAN-1 and COLO-357) under the stimulation with IL-1α (10 μg/L) or IL-6 (100 μg/L).Results Northern blot analysis revealed the presence of the 4.1 kb VEGF-A mRNA transcript and 2.4 kb VEGF-C mRNA transcript in all 6 tested cell lines.Immunoblotting with highly specific anti-VEGF-A,anti-VEGF-C antibody revealed the presence of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines.RT-PCR analysis revealed the levels of VEGF-A and VEGF-C gene were 1-2 fold and 1 fold increase in COLO-357 cell line by stimulation with IL-1α,however,no effect was found in CAPAN-1 cell line.The levels of VEGF-A and VEGF-C gene were 2-5 fold and 1 fold increase in CAPAN-1 cell line by stimulation with IL-6,but no effect was found in COLO-357 cell line.Conclusion The interaction between VEGF-A,-C and these cytokines in pancreatic cancer contributes to the distant and lymphatic metastasis and this disease progression.

关 键 词：分泌血管内皮生长因子 白细胞介素-1Α Western杂交法 调节作用 NORTHERN杂交 IL-1α 人胰腺癌细胞株 聚合酶链式反应 VEGF-A 细胞活素 刺激作用 IL-6 癌细胞产生 C基因 PCR) 淋巴转移 相互作用 蛋白质 分子量 基因分 MIA 逆转录 A基因 显示 分析法 

分 类 号：R735.9[医药卫生—肿瘤]