白细胞介素-12抑制裸鼠肝癌Ang-2和血管内皮生长因子表达及其意义  

Interleukin-12 inhibiting the expression of Ang-2 and VEGF gene in hepatocarcinoma of nude and its significance

作　　者：潘运龙[1] 覃莉[2] 

机构地区：[1]暨南大学附属第一医院外科,广州510632 [2]暨南大学医学院组织胚胎学教研室

出　　处：《中华实验外科杂志》2005年第4期439-441,共3页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金资助项目 (0 1 0 367)

摘　　要：目的　探讨白细胞介素 12抑制裸鼠肝癌中血管生成素 2 (Ang 2 )和血管内皮生长因子(VEGF)基因表达及其意义。方法　将小鼠白细胞介素 12基因转染小鼠肝癌H 2 2细胞后,接种到裸鼠肾包膜下,第11天取出肿瘤组织。通过逆转录 聚合酶链反应(RT PCR)观察实验组和对照组瘤组织中Ang 2和VEGF基因在mRNA水平上的差异。结果　实验组Ang 2和VEGF基因的PCR产物条带亮度均弱于对照组(P <0 .0 5 ) ,实验组Ang 2 (0 .7878±0 .14 71)和VEGF(0 .8867±0 .192 4)基因的PCR产物条带辉度比值低于载体对照组(1.0 3 19±0 .15 74、1.1744±0 .1189)和空白对照组(1.0 3 61±0 .15 3 6、1.1874±0 .175 2 ,P <0 .0 5 )。结论　IL 12抑制Ang 2和VEGF基因在裸鼠肝癌组织中的表达。Objective To explore the interleukin-12 inhibiting the expression of angiopoietin-2 (Ang-2) and vascular endothelial growth factor (VEGF) gene in hepatocarcinoma of nude and its significance.Methods The mouse interleukin-12 gene was transfected into H22 cells,hepatocarcinoma cells of mouse.Then they were inoculated into subrenal capsule of the nude mice.The tumors were taken out on the 11th day.The difference at the level of mRNA of gene of Ang-2 and VEGF was observed by RT-PCR between the experimental group and control group.Results The brightness of Ang-2 and VEGF gene in the experimental group was weaker than that in control group ( P < 0.05). The luminance ratio of Ang-2 (0.787?8± 0.147?1) and VEGF (0.886?7± 0.192?4) gene in the experimental group was lower than that in the vector control group (1.031?9± 0.157?4, 1.174?4± 0.118?9) and the blank control group (1.036?1± 0.153?6, 1.187?4± 0.175?2, P < 0.05). Conclusion IL-12 can inhibit the expression of Ang-2 and VEGF gene in hepatocarcinoma tissue and the angiogenesis of hepatocarcinoma in nude mice.

关 键 词：ANG-2 其意义 血管内皮生长因子(VEGF) 裸鼠 抑制 小鼠白细胞介素-12 逆转录-聚合酶链反应 VEGF基因 PCR产物 mRNA水平 基因表达及 血管生成素 H22细胞 IL-12 对照组 实验组 小鼠肝癌 基因转染 肾包膜下 肿瘤组织 PCR) 肝癌组织 

分 类 号：R735.7[医药卫生—肿瘤]