反义转化生长因子-β1基因对骨肉瘤细胞表达转移相关因子的影响  

Effects of antisense transforming growth factor-β1 on the factors related to metastasis in osteosarcoma

作　　者：鲍同柱[1] 赵红卫[1] 郑启新[2] 刘万军[1] 刘勇[2] 

机构地区：[1]三峡大学第一临床医学院湖北省宜昌市中心人民医院骨科,湖北宜昌443003 [2]华中科技大学同济医学院附属协和医院骨科

出　　处：《中华实验外科杂志》2005年第4期477-478,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　探讨反义转化生长因子(TGF) β1基因转染对骨肉瘤细胞MG 63表达基质金属蛋白酶(MMPs)和尿激酶型纤溶酶原激活因子(uPA )等肿瘤转移相关因子的影响。方法　将反义TGF β1基因转染MG 63 ,G418筛选转染细胞后,采用Northernblot法检测转染细胞MMP 2、MMP 9和uPA的表达情况。结果　反义TGF β1基因转染骨肉瘤细胞上清培养的Mv 1 Lu细胞增殖活性为0 .975±0 .0 16(对照组1.0 3 2±0 .0 2 7,P >0 .0 5 ) ;Northernblot法检测显示MMP 2和uPAmRNA的表达明显降低。结论　通过反义基因技术阻断骨肉瘤细胞TGF β1自分泌环,在降低肿瘤细胞TGF β1表达的同时,也降低了转移相关因子MMP 2、uPA的表达。Objective To investigate the effects of antisense transforming growth factor-β1 on the factors related to metastasis in osteosarcoma.Methods The osteosarcoma cells were transfected by antisense TGF-β1 gene and selected by G418.Northern blot was used to detect the expression of MMP-2, MMP-9 and uPA mRNA in the transfected cells.Results The proliferation activity of Mv-1-Lu that were cultured in supernatant of transfected osteosarcoma cells was 0.975± 0.016 (control group 1.032± 0.027, P > 0.05). The expression of MMP-2 and uPA mRNA in the transfected cells was decreased markedly.Conclusion To block the TGF-β1 autocrine loop of osteosarcoma cells through antisense gene technology could suppress the expression of MMP-2 and uPA,which can inhibit the invasion and metastasis of tumor.

关 键 词：反义转化生长因子-β1基因 转移相关因子 细胞表达 反义TGF-β1 Northem 纤溶酶原激活因子 TGF-β1表达 MMP-2 基质金属蛋白酶 骨肉瘤细胞 BLOT法 基因转染 细胞增殖活性 转染细胞 MMP-9 侵袭和转移 uPA 尿激酶型 表达情况 细胞上清 mRNA 自分泌环 基因技术 肿 

分 类 号：R738.1[医药卫生—肿瘤] R734.207[医药卫生—临床医学]