抗菌多肽葛佬素的原核表达纯化及活性鉴定  

Prokaryotic expression, purification and identification of anti-bacterial peptide gloverin

作　　者：许波[1] 周红[2] 李慧增[1] 鲁永玲[2] 王良喜[2] 丁国富[2] 

机构地区：[1]第三军医大学西南医院口腔科,重庆400038 [2]第三军医大学基础医学部药理学教研室,重庆400038

出　　处：《第三军医大学学报》2005年第6期529-531,共3页Journal of Third Military Medical University

基　　金：中关村科技生物医药创新研究基金(2001)~~

摘　　要：目的　原核表达抗菌肽葛佬素,并对其生物学活性进行初步鉴定。方法　应用pET原核表达系统进行目的 蛋白的表达,应用蛋白免疫印迹对蛋白进行鉴定,并采用鲎试剂法,对表达产物的生物活性进行初步鉴定。结果　体外表 达产物经蛋白免疫印迹鉴定,为融合有聚组氨酸标签的葛佬素抗菌肽,鲎试剂检测结果表明,该表达产物对LPS有明显的 中和活性。结论　在原核表达系统中成功表达了具有生物活性的抗菌肽葛佬素。Objective To express anti-bacterial peptide gloverin in prokaryotic system and investigate its bioactivity. Methods E.coli BL21 was used to express gloverin. Western blot was used to identify the production and Tachypleus Amebocyte Lysate (TAL) was used to detect the bioactivity of the production. Results The production was identified as gloverin peptide by Western blotting, and the TAL data indicated that the production could neutralize LPS. Conclusion Anti-bacterial peptide gloverin is successfully expressed in E.coli BL21.

关 键 词：葛佬素 抗菌肽 原核表达 内毒素 

分 类 号：R394.3[医药卫生—医学遗传学] R977.6[医药卫生—基础医学]