新型毛细管等电聚焦驱动方法的建立及其应用  

Construction of a New Method for Mobilizing the Zones of CIEF and Its Application

作　　者：朱贵杰[1] 杨春[1] 刘和春[1] 张丽华[1] 张维冰[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所,国家色谱研究分析中心,大连116011

出　　处：《高等学校化学学报》2005年第4期654-656,共3页Chemical Journal of Chinese Universities

基　　金：国家"九七三"计划项目(批准号:001CB510202)资助.

摘　　要：A simple method for mobilizing the focused zones of capillary isoelectric focusing(CIEF) for single-point detectors was developed, in which a linear polyacrylamide(LPA) coated capillary was connected with an uncoated capillary by an etched porous joint. The etched section allows electrical conductivity but blocks the hydrodynamic flow. The focused zones of mixed proteins was mobilized by this method successfully. Compared with the traditional gravity mobilization step, it was found that this method can mobilize the focused zones more effectively without sacrificing separation efficiency.A simple method for mobilizing the focused zones of capillary isoelectric focusing(CIEF) for single-point detectors was developed, in which a linear polyacrylamide(LPA) coated capillary was connected with an uncoated capillary by an etched porous joint. The etched section allows electrical conductivity but blocks the hydrodynamic flow. The focused zones of mixed proteins was mobilized by this method successfully. Compared with the traditional gravity mobilization step, it was found that this method can mobilize the focused zones more effectively without sacrificing separation efficiency.

关 键 词：毛细管等电聚焦 电渗流驱动 蛋白质 多肽 

分 类 号：O658[理学—分析化学]