重组人白介素-2鉴别试验方法的研究  

Research of Distinctive Test in IL-2

作　　者：李英华[1] 尹红志[1] 徐福平[1] 许国杰[1] 郭德本[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《中国地方病防治》2005年第2期82-83,共2页Chinese Journal of Control of Endemic Diseases

摘　　要：目的　比较白介素-2鉴别试验3种方法的特点。方法　应用免疫印染法、免疫斑点法和琼脂双扩散法对白介素-2进行鉴别试验。结果　3种鉴别试验结果基本一致。其中免疫印染法的灵敏度最高、特异性最强,适合微量分析;琼脂双扩散法操作最简便,适合基层单位定性分析。结论　为选择合适的鉴别试验提供了实验依据。Objective Characteristic of three methods were compared by Distinctive test in IL-2.Methods Distinctive test in IL-2 were done by western blot-immune dot double-spreaded agar method.Results The result is elementary similar.In this three methods,western bolt is the most sensitive and particular so it is fit for microquantity,double-spreaded-agar method is the most simple so it is fit for basic unit in qualitative analysis.Conclusions It provides laboratory evidence in order to choose suitable distinctive test.

关 键 词：鉴别试验方法 重组人白介素 白介素-2 双扩散法 免疫斑点法 方法应用 试验结果 微量分析 定性分析 基层单位 种鉴别 灵敏度 特异性 印染 琼脂 

分 类 号：R927.1[医药卫生—药学] R457.7