脱氧雪腐镰刀菌烯醇对人外周血单个核细胞TAP-1表达的抑制作用  

The inhibitory effect of deoxynivalenol on TAP-1 expression in human peripheral blood mononuclear cells in vitro

作　　者：李月红[1] 张祥宏[1] 邢凌霄[1] 左连富[2] 严霞[1] 王俊灵[1] 王凤荣[1] 

机构地区：[1]河北医科大学实验病理室,河北石家庄050017 [2]河北省肿瘤研究所细胞室,河北石家庄050011

出　　处：《细胞与分子免疫学杂志》2005年第2期246-248,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：河北省自然科学基金资助项目 (No. 301350 );科技部重大基础研究项目前期研究专项资助(No. 2001CCC00500)

摘　　要：　目的: 探讨脱氧雪腐镰刀菌烯醇 (DON)对人外周血单个核细胞 (PBMC)中TAP- 1表达的影响。方法: 采用流式细胞仪(FCM)和半定量RT- PCR方法, 从蛋白和mRNA水平上分析不同浓度的DON对体外培养的人PBMC中TAP- 1分子表达的影响及量 效关系。结果: FCM定量检测表明, 用不同浓度的DON处理, 均可一定程度地抑制人PBMC中TAP -1蛋白的表达, 且二者呈显著的负相关 (r= -0. 865,P<0. 01)。半定量RT -PCR检测显示, 不同浓度的DON处理均可抑制人PBMC中TAP -1mRNA的表达。结论: DON可剂量依赖地抑制体外培养的人PBMC中TAP- 1蛋白和其mRNA的表达, 对阐明食管癌高发区被DON污染的粮食与食管癌发生的关系具有重要的意义。AIM: To explore the effects of deoxynivalenol (DON) on transporter associated with antigen processing-1 (TAP-1) expression in human peripheral blood mononuclear cells (PBMCs). METHODS: Effects of various concentrations of DON on TAP-1 expression of in vitro cultured human PBMCs and the dose-effect relationship were analyzed by flow cytometry (FCM) and semi-quantitative RT-PCR at the protein and mRNA levels. RESULTS: FCM analysis indicated that treatment with various concentrations of DON could inhibit TAP-1 expressions in PBMCs, showing a negative correlation between DON concentration and TAP-1 expression. Semi-quantitative RT-PCR detection indicated that high concentrations of DON (1 000 and 2 000 μg/L) could distinctly inhibit TAP-1 mRNA expression. CONCLUSION: DON can down-regulate TAP-1 expression in human PBMCs in a dose-dependent manner in vitro, which suggests DON may interfere with host immunosurveillance, and this may account for the correlation between DON-contaminated grain and esophagus cancer in high risk area.

关 键 词：脱氧雪腐镰刀菌烯醇 人外周血单个核细胞 TAP-1 RT—PCR 

分 类 号：R378[医药卫生—病原生物学]