质疑血清药理学,加强多层次半体内实验研究  

Doubt the validity of serum pharmacology, emphasize the ex vivo experiments on multiple levels

作　　者：贺石林[1] 葛金文[2] 贺蓉[1] 梅志刚[2] 

机构地区：[1]中南大学湘雅医学院止血生理实验室 [2]湖南中医学院血管生物实验室,湖南长沙410078

出　　处：《中国药理学通报》2005年第3期277-279,共3页Chinese Pharmacological Bulletin

摘　　要：质疑血清药理学的主要理由包括①从胃肠吸收的中药成分在体内是进入血浆,而不是血清;②凝血过程有一系列酶生成,同时刺激白细胞释放溶酶,这些酶可能降解某些中药成分;③凝血酶刺激血小板与白细胞释放大量生物活性物质;④凝血过程伴有纤溶、补体、激肽等系统活化;⑤制备与灭活血清过程会导致中药成分与中药介导体内释放物质损失。新近利用HPLCMS指纹图谱分析与抗白血病细胞增殖实验证明利用血清药理方法可能带来错误结论。建议加强中药多层次半体内实验研究,其中血浆药理方法可能避免血清药理方法的弊病。The main reasons to doubt the validity of serum pharmacology include:①The components of medical herbs absorbed from gastrointetinal tract enter circulating plasma,not serum in vivo.②Blood coagulation involves the activation of a series of proenzymes,and the proteases induce leukocytes to release lysosomal enzymes.These enzymes may degrade some herbal components.③Thrombin can stimulate platelets and leukocytes to secrete many biologically active substances.④Fibrinolysis,complement and kinin systems are activated during blood coagulation.⑤The processes of serum preparation and inactivation always cause lose of lots of herbal components. Recently finger printing analysis using HPLC-MS and antileukemic proliferation experiments showed that the results obtained from serum pharmacological method might be incorrect. We propose that the ex vivo experiments on multiple levels are necessary to elucidate the pharmacological actions and their mechanisms of medical herbs and believe that the pharmacological method using plasma absorbed herbal components should be better than that using serum.

关 键 词：中药成分 血清药理方法 血浆 药理作用 

分 类 号：R-05[医药卫生] R282.71