番茄红素对胃癌细胞SGC-7901生长的抑制作用  

The inhibitory effect of lycopene on growth of gastric carcinoma cells SGC-7901

作　　者：张卫国[1] 谢国建[1] 刘先军[1] 吴清明[1] 王小虎[1] 于皆平[2] 

机构地区：[1]郧阳医学院附属十堰市太和医院消化内科,湖北十堰442000 [2]武汉大学人民医院消化内科,湖北武汉430060

出　　处：《中国药理学通报》2005年第3期292-295,共4页Chinese Pharmacological Bulletin

基　　金：郧阳医学院附属太和医院重点科研资助项目(No025)

摘　　要：目的研究番茄红素体外对胃癌细胞SGC7901生长的抑制作用。方法将番茄红素作用于胃癌细胞SGC7901,四甲基偶氮唑蓝(MTT)法测定细胞生长抑制率,3H胸腺嘧啶核苷(3HTdR)参入试验测定对细胞DNA合成的影响,TRAPPCRELISA法检测端粒酶活性,流式细胞仪检测细胞周期及凋亡。结果番茄红素作用后胃癌细胞生长明显受抑制,24、48、72和96h的IC50分别为22×10-4、15×10-4、67×10-6和40×10-6mmolL-1。10-5mmolL-1的番茄红素作用胃癌细胞24、48、72、96h后,3HTdR掺入量分别为(442512±13409)cpm、(365647±15391)cpm、(275365±12833)cpm、(161482±9456)cpm,低于对照组的(624831±26563)cpm、(750582±25898)cpm、(832124±22352)cpm、(859130±36821)cpm(P<001);TRAPPCRELISA法显示A值分别为1102±0026、0846±0018、0735±0017、0586±0014,低于对照组(1801±0048、1887±0072、2047±0085、2131±0076,P<001)。10-5mmol·L-1的番茄红素作用胃癌细胞96h后,G0/G1期细胞比例为(697±52)%,高于对照组的(456±37)%(P<001),凋亡率达(256±28)%。结论番茄红素能明显抑制胃癌细胞增殖及端粒酶活性,能使细胞产生G0/G1期阻滞,并诱导凋亡。Aim To investigate the inhibitory effect of lycopene on growth of gastric carcinoma cells SGC-7901.Methods The gastric carcinoma cells SGC-7901 were treated with lycopene. The rate of growth suppression on cells was evaluated by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The effect of lycopene on DNA synthesis of gastric carcinoma cells was evaluated by ~ 3H-thymidine (~ 3H-TdR) incorporation. The activity of telom-erase was examined by telomeric repeat amplification protocol (TRAP) PCR-ELISA. Cell cycle and apoptosis were detected by flow cytometry (FCM).Results Lycopene had obvious inhibitory effect on growth of gastric carcinoma cells, IC_~50 of 24, 48, 72 and 96 h were 2.2×10^-4 , 1.5×10^-4 ,6.7×10^-6 and 4.0×10^-6 μmol·L^-1 respectively.Treated with 10^-5 mmol·L^-1 lycopene for 24, 48, 72 and 96 h, ~ 3H-TdR incorporation of gastric carcinoma cells were (4 425.12±134.09) cpm, (3 656.47±153.91) cpm, (2 753.65±128.33) cpm and (1 614.82±94.56) cpm respectively which showed significant decrease(P<0.01), as compared with that in control group which were (6 248.31±265.63) cpm, (7 505.82±258.98) cpm, (8 321.24±223.52) cpm and (8 591.30±368.21) cpm respectively; light absorption (value A) of gastric carcinoma cells (1.102±0.026、0.846±0.018、0.735±0.017 and 0.586±0.014) showed significant decrease(P<0.01), as compared with that in control group (1.801±0.048, 1.887±0.072, 2.047±0.085 and 2.131±0.076). Treated with 10^-5 mmol.L^-1 lycopene for 96 h, the ratio of G_0/G_1 phase of gastric carcinoma cells was(69.7±5.2)% which showed increase (P<0.01), as compared with that in control group which was(45.6±3.7)%; the rate of apoptotic cells reached (25.6±2.8)%. Conclusions Lycopene can significantly inhibit proliferation and activity of telomerase of gastric carcinoma cells.It can cause G_0/G_1 blocking and induce apoptosis also.

关 键 词：番茄红素 胃癌细胞 端粒酶 细胞周期 凋亡 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]