γ射线照射对前列腺癌细胞金属基质蛋白酶和促红细胞生成素受体表达的影响  

Effects of matrix metalloproteinase and erythropoietin-receptors by 60Co γ-ray external irradiation of human prostate cancer PC-3 cells

作　　者：张晓毅[1] 洪宝发[1] 周立权[1] 周建光[2] 邹跃[3] 邹练[3] 

机构地区：[1]中国人民解放军总医院泌尿外科 [2]军事医学科学院,北京市100071 [3]中国人民解放军第二炮兵总医院,北京市100088

出　　处：《实用医学杂志》2005年第7期665-667,共3页The Journal of Practical Medicine

摘　　要：目的:前列腺癌PC-3细胞接受不同剂量γ射线照射后,观察其金属基质蛋白酶-2(M M P2)、金属基质蛋白酶-9(M M P9)及促红细胞生成素受体蛋白(E PO-R)表达的变化,阐明γ射线对前列腺癌PC-3细胞生物学行为的影响。方法:分别用1G y、3G y、5G y剂量的60C oγ射线,照射体外培养的PC-3细胞系。用流式细胞术检测M M P2、M M P9及E PO-R的含量;并测定细胞生长曲线及克隆形成率。结果:(1)1G y剂量即可明显抑制细胞的生长,并且细胞克隆形成率随着剂量的增加而降低;(2)E PO-R表达在1G y和3G y组高于对照组,5G y组低于对照组;(3)M M P2总体上出现降低趋势;M M P9表达在1～5G y组均高于对照组。结论:虽然60C oγ射线照射可明显抑制前列腺癌PC-3细胞的生长,但是M M P9及E PO-R表达却增高,提示亚致死剂量的γ射线照射可使存活细胞的浸润性增高。Objective To study the changes of the matrix metalloproteinases 2(MMP2),matrix metalloproteinases 9(MMP9) and erythropoietin receptors(EPO R) induced by 60Co γ rays external irradiation of human prostate cancer PC 3 cells. Methods Human prostate cancer PC 3 cell lines was irradiated with different dose of 60Co γ rays. Cell migration was evaluated. The expression of MMP2,MMP9 and EPO R were investigated by flow cytometry (FCM). Results Irradiation enhances invasive potential at the dose of 1 Gy and 3 Gy,where as it significantly inhibits cell growth. Conclusion The different doses of 60Co γ rays external irradiation for prostate cancer may have different effects through the changes of MMP2,MMP9 and EPO R.

关 键 词：金属基质蛋白酶 促红细胞生成素 前列腺癌细胞 受体表达 ^60COΓ射线照射 PC-3细胞 细胞生物学行为 流式细胞术检测 细胞克隆形成率 MMP9 细胞生长曲线 亚致死剂量 对照组 不同剂量 受体蛋白 体外培养 MMP2 抑制细胞 存活细胞 

分 类 号：R737.25[医药卫生—肿瘤]