乳腺癌BRCA1蛋白表达与DNA定量分析的临床研究  

BRCA1 protein and the content of DNA in the sporadic breast cancer patients in Liuzhou

作　　者：赵时梅[1] 姚波[1] 严龙[1] 黄玲[1] 陈琼[1] 黄武国[1] 林海兰[1] 

机构地区：[1]广西柳州市中医院,柳州545001

出　　处：《广西医学》2005年第3期325-329,共5页Guangxi Medical Journal

基　　金：广西柳州市科技局立项课题 (合同编号 :2 0 0 3自 0 2 1 8)

摘　　要：目的　研究柳州地区人散发性乳腺癌BRCA1蛋白及mRNA表达与DNA含量的关系及意义。方法　应用S P免疫组化法及图像分析检测2 5例正常乳腺上皮和增生上皮及5 0例散发性乳腺癌组织中BRCA1蛋白表达及DNA情况。应用原位杂交技术检测5 0例乳腺癌中的2 0例乳腺癌组织BRCA1mRNA表达。结果　在乳腺正常上皮、增生上皮和癌中BRCA1表达及DNA含量差异均具有显著性(P <0 . 0 1) ;BRCA1表达与DNA含量呈负相关(P <0. 0 1) ;BRCA1在不同肿瘤大小和不同组织学类型中的表达差异无显著性(P >0 . 0 5 ) ,但在不同组织分级和有无淋巴结转移中的表达差异有显著性(P <0 . 0 5 )。DNA分析中,DI值和>5C %值总体分布在不同组织学类型中差异无显著性(P >0. 0 5 ) ,但在不同肿瘤大小、不同组织分级和有无淋巴结转移中差异有显著性(P <0 . 0 5 )。结论　乳腺上皮中DNA含量增加,BRCA1表达下降,对散发性乳腺癌的发生发展具有一定作用。检测BRCA1表达及DNA含量在散发性乳腺癌的早期诊断、判断生物学行为、预测预后中有重要意义。Objective To study the relations and significance between the expression of BRCA1 protein and mRNA and the content of DNA in the sporadic breast cancer tissue of the peoples in Liuzhou area.Methods Immunohistochemistry technique and image analysis were used to detect the expression of BRCA1 protein and content of DNA in 25 cases normal mammary epithelium and proliferative epithelium and 50 cases breast cancer tissues.Hybridization in situ was used to detect the expression of BRCA1 mRNA in 20/50 cases breast cancer tissues.Result 1.The expression of BRCA1 and DNA content in normal breast epithelium, and in lightly, highly proliferative epithelium and in atypical hyperplasia breast epithelium and in cancer tissues show significant differences (P<0.01).2.The expression of BRCA1 and content of DNA show negative correlation(P<0.01).3.The expression of BRCA1 show indifferences in different size and histology type of tumour (P>0.05),but show differences in different tissue grade and lymph metastasis and nonmetastasis (P<0.05).In DNA analysis,DI titer and >5C% titer total distribution show indifferences in different histology type of tumour(P>0.05),but show difference in different size of tumour and in different tissue degree and lymph metastasis and unmetastasis (P<0.05).Conclusions The increase of DNA content and the decrease of BRCA1 expression play some roles in the occurrence and development of breast carcinoma.Detecting BRCA1 expression and DNA content have important significance in earlier diagnosis,judgement of biological behavior,prediction prognosis of breast carcinoma.

关 键 词：散发性乳腺癌 BRCA1 DNA含量 免疫组织化学 原位杂交 图像分析 

分 类 号：R738[医药卫生—肿瘤]