我国汉族和彝族rRNA基因多态性的研究  

Study on Polymorphism of Ribosomal RNA Gene in Chinese Hans and Yis

作　　者：袁飒英 蒋伟宏[1] 陆嫣[1] 费虹明[1] 陈仁彪[1] 

机构地区：[1]上海第二医科大学生物学教研室,中科院上海细胞生物学研究所

出　　处：《Acta Genetica Sinica》1994年第3期173-178,共6页

摘　　要：人类ｒＤＮＡ顺序既包含保守性程度高的转录区，又有变异性较大的不转录间隔区。随着对ｒＤＮＡ结构的不断认识，发现对不转录间隔区特征的研究在分子群体遗传学上具有重要的价值。我们运用ｒＤＮＡ基因探针和Ｓｏｕｔｈｅｒｎ印迹法对我国汉族和彝族ｒＲＮＡ基因３＇端不转录间隔区作多态性分析，ＲＦＬＰ特征揭示两个民族有广泛的共性，又有各自的特点。In the secondary constriction region of the short arm of human group D and group G acrocentric chromosomes there are tandemly repeated sequences of transcriptable segments and non-transcriptable spacers of rRNA genes.In the non-transcriptable spacer segments there is a polymorphic region with length variation. This paper reports the results obtained when the BamHI digested products of genomic DNA from 36 cases of Han nationality and those from 40 cases of Yi nationality were probed with a cDNA subprobe,pABE,which corresponds to the downstream one third of the 28S rRNA gene and is 1.6kb long with BamHI and EcoRI restriction sites at its two terminnal respectively,using Southern blotting and molecular hybridization techniques.A total of 10 different restriction fragments were detected.Four fragments(7.9,4.9,4.0 and 1.2 kb)were seen in all subjects tested and the frequencies of six other polymorphic fragments in Hans and Yis(10.0,9.4,8.5,6.7,6.0 and 3.0 kb)showed no significant difference when compared to each other.From the 10 different patterns of combination of restriction fragments four of them(1HY,2HY,3HY and 4HY)were detected in both Hans and Yis with no significant difference between their respective frequencies. Four other patterns(5H,6H,7H and 8H)were only seen in Hans with individual frequencies not high enough to be statistically significant.However,the statistical test for the combined frequencies of these four patterns showed highly significant difference between Hans and Yis(P<0.01).The last two patterns(9Y and 10Y)were only seen in Yis with frequency of 9Y being significantly different from the zero distribution in Hans.The difference becomes highly significant(P<0.01)when the combined frequency forthese two patterns were compared to the zero distribution in Hans.The length polymorphism of the non-transcriptable spacer of the rRNA genes is very probably originated from unequal crossing-over events.The results reported here confirm that this length polymorphism is a useful genetic marker for the studi

关 键 词：汉族 彝族 RFLP RRNA 基因 多态性 

分 类 号：Q987[生物学—遗传学]