胃癌中E-cadherin基因5’端CpG岛甲基化研究  

Methylation of Gene E-cadherin 5’CpG Island in Gastric Carcinomas

作　　者：赵英芳[1] 田新霞[2] 丁鑫[1] 

机构地区：[1]内蒙古科技大学基础医学部病理学教研室,内蒙古包头014010 [2]北京大学医学部病理学系

出　　处：《包头医学院学报》2005年第1期7-9,共3页Journal of Baotou Medical College

摘　　要：目的:检测胃癌组织中E-cadherin基因 5’端CpG岛启动子区域的甲基化状况,探讨该基因甲基化与胃癌组织学类型、淋巴结转移、年龄、性别之间的关系,研究该基因甲基化在胃癌发生发展过程中的作用。方法:采用甲基化特异性PCR方法对胃黏膜中E-cadherin基因 5’端CpG岛启动子区域甲基化状况进行研究。结果:胃癌中E-cadherin基因甲基化率为 34. 6% (28 /81),而正常胃黏膜中均未检测到该基因异常甲基化。E-cadherin基因甲基化与肿瘤组织学类型、淋巴结转移及性别均无相关性,但与年龄有关,随着年龄增加患者甲基化率呈增高趋势。结论:E-cadherin基因 5’端CpG岛启动子区域甲基化与年龄有关。E-cadherin基因 5’端CpG岛启动子区域异常甲基化是胃癌细胞常见的分子事件,可能参与了胃癌的发生发展过程。Objective:To investigate the promoter methylation status of gene E-cadherin in gastric carcinoma, and its correlation with clinical and pathological parameters. Methods:A series of 81 gastric carcinomas and 10 cases of normal gastric tissues were examined for the promoter methylation of E-cadherin by methylation-specific PCR method. Results:Methylation of E-cadherin was found in 34.6% (28/81) of gastric carcinomas, but was not found in the normal gastric mucous membrane.Promoter methylation of E-cadherin was not associated with histological type of the tumor,lymph node metastasis and gender but correlated with age.There is a tendency of increase in methylation with the increase in age.Conclusion:Methylation of E-cadherin is related to age,which is a common molecule event in gastric carcinomas and may play a role in the development and progression of gastric carcinomas.

关 键 词：胃肿瘤病理学 DNA甲基化 E-CADHERIN基因 

分 类 号：R735.2[医药卫生—肿瘤]