磁性高分子微球固定化中性蛋白酶的研究  

Immobolization of Neutral Protease onto Magnetic Polymer Microspheres

作　　者：李孝红[1] 章贤明[1] 丁小斌[1] 孙宗华[1] 

机构地区：[1]中国科学院成都有机化学研究所

出　　处：《应用化学》1994年第5期1-4,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金资助项目

摘　　要：以表面带羟基的磁性高分子微球为载体，对位苯醌活化后，通过共价结合修饰中性蛋白酶，得到比活性为１０００Ｕ／ｇ的磁性固定化酶。偶联蛋白量２０～３０ｍｇ／ｇ载体，固定化酶活性保持达４０％，自由酶和固定化酶相比，最适温度从５０℃变到５０～６０℃，最适ｐＨ从７．５变到６．５，Ｋｍ从０．０５４％变到０．０８８％酪蛋白溶液，ｐＨ稳定性、热稳定性、贮存稳定性都有较大提高．A neutral protease from Balilius Subtilis has been immobilized onto the magneticpolymer microspheres,which were obtained from styrene,hydroxyethyl methacrylate,di-vinyl benzene and Fe_3O_4,and had hydroxyl groups in the surface by covalent coupling.Themagnetic immobilized neutral protease had a enzyme activity of 1000U/g,enzyme yield of20～30mg/g carrier,and activity retention of about 40%.For both free enzyme and immobi-lized enzyme the optimum work temperatures were 50℃ and 50～60℃,the optimum pHvalues were 7.5 and 6.5, and the kinetic constants were 0.054%and 0.088%,respectively.The thermal stability, pH stability,and the storage stability of the immobilized enzyme areimproved obviously.

关 键 词：高分子微球 固定化 中性蛋白酶 

分 类 号：O629.8[理学—有机化学]