HPLC法测定灌胃当归胡椒复方小鼠血浆中阿魏酸的含量  

Determination of ferulic acid in plasma of mice plasma administered with Danggui Hujiao Decoction by HPLC

作　　者：裴瑾[1] 杨祖贻[2] 刘荣敏[2] 陈佳[2] 万德光[1] 胡荣[1] 

机构地区：[1]成都中医药大学药学院,四川成都610075 [2]四川省肿瘤研究所,四川成都610041

出　　处：《中草药》2005年第4期523-525,共3页Chinese Traditional and Herbal Drugs

基　　金：国家中医药管理局科研基金资助项目(02-03ZP50);四川省中医药管理局科研基金资助项目(200217)

摘　　要：目的建立灌胃当归胡椒复方小鼠的血浆中阿魏酸的分析方法。方法采用HPLC法,以甲醇-水-冰醋酸(36.4∶63∶0.6)为流动相,色谱柱KromasilC18(250mm×4.6mm,7μm)为固定相,体积流量1.0mL/min,紫外检测波长322nm。采用外标法定量。结果阿魏酸与血浆中其他组分能很好分离,阿魏酸在1.88～188.00ng/μL线性关系良好(r=0.999),最低检测浓度0.47ng/μL,平均回收率为94.85%。结论本方法具有准确、灵敏、专一性高等特点,适于阿魏酸的血药浓度测定及药动学研究。Objective To establish an analytical method for determination of ferulic acid inDanggui HujiaoRadix Angelicae Sinensis and Fructus Piperis]) Decoction in mouse plasma.Methods HPLC method was used.The conditions of chromatography: Kromasil C 18250 mm×4.6 mm, 7 μm) was used with a mobile phase of CH3OH-H2O-CH3COOH (36.4∶63∶0.6).Flow rate was 1.0 mL/min.The detecting wavelength was 322 nm.External standard method was quantitative analysis method.Results The ferulic acid could be totally separated from other ingredients in plasma.The linear range was 1.88—188.00 ng/μL (r=0.999), the lowest detectability was 0.47 ng/μL, and the average recovery was 94.85%.Conclusion This method provides an accurate and sensitive way in detecting blood concentration of ferulic acid and studying in pharmacokinetics.

关 键 词：当归胡椒复方 阿魏酸 高效液相色谱 血药浓度 

分 类 号：R286.02[医药卫生—中药学]