胃癌患者外周血中CK19,CK20表达的临床研究  

Clinical Study on Expression of Cytokeratin 19, Cytokeratin 20 in Peripheric Blood of Patients with Gastric Cancer

作　　者：何成全[1] 詹乾钢[1] 单水阳[1] 胡伯年[1] 叶平江[1] 冯刚[1] 许焕建[1] 付文安[1] 

机构地区：[1]绍兴市人民医院肿瘤科,浙江省绍兴市312000

出　　处：《中国肿瘤临床》2005年第7期401-403,共3页Chinese Journal of Clinical Oncology

摘　　要：目的:研究胃癌患者外周血CK19、CK20的表达,并探讨其临床意义。方法:用流式细胞术(FCM)方法,检测66例胃癌、7例消化系统良性疾病及21例本院健康职工外周血CK19、CK20的表达。结果:7例消化系统良性患者、21例本院健康职工外周血CK19、CK20无表达。66例胃癌患者CK19、CK20、CK19+CK20阳性表达率分别为53.1%(35/66)、56.1%(37/66)、46.9%(31/66)。TNM分期之间比较,Ⅳ期与Ⅰ、Ⅱ、Ⅲ期差异有显著性意义(P<0.01)。Ⅰ、Ⅱ、Ⅲ期之间相互比较,差异无显著性意义(P>0.05)。转移程度比较,差异有显著性意义(P<0.01)。胃癌远处转移诊断敏感度高达90%。结论:胃癌患者外周血CK19、CK20可以作为检测胃癌微转移的指标。Objective: To study the expression of cytokeratin 19(CK 19), cytokeratin 20 (CK 20) in periphery blood of patients with gastric cancer and its clinical significance. Methods: The expressions of CK19, CK20 in periphery blood samples from 66 patients with gastric cancer, 7 patients with benign diseases of digestive system and 21 healthy volunteers were detected by with FCM. Results: In blood samples from 7 patients with benign digestive diseases and 21 healthy volunteers, no expression of CK19, CK20 was detected (0/28). In blood sample from 66 patients with gastric cancer, positive expression rate of CK19 was 53.1% (35/66), positive expression of CK20 was 56.1% (37/66), positive expression of CK19 and CK20 was 46.9% (31/66). A significant difference (P<0.01) was found in the expression of CK19 and of CK20 between samples of patients with stage Ⅳ and Ⅰ～Ⅲ(TNM method). No significant difference was found in samples of patients with stageⅠ～Ⅲ. The difference was significant between samples of patients with distant metastasis and no metastasis. The diagnosis of metastasis is high sensitivity (90%) and low specificity (60.71%). Conclusion: The Expression of CK19 and CK20 in periphery blood of patients with gastric cancer may be helpful in the selection of clinical treat and judgment of prognosis.

关 键 词：胃癌 微转移 CK19 CK20 流式细胞术 

分 类 号：R735.2[医药卫生—肿瘤]