以GFP为报告基因的酿酒酵母mRNA在哺乳动物细胞中的表达  

The expression of green fluorescent protein gene mRNA from yeast in mammalian cell

作　　者：王弘[1] 郑文岭[2] 杨太成[2] 冼江[2] 马文丽[3] 

机构地区：[1]华南农业大学食品学院,广州510640 [2]广州军区广州总医院医学实验科,广州510010 [3]南方医科大学分子生物研究所,广州510515

出　　处：《广东医学》2005年第4期449-451,共3页Guangdong Medical Journal

基　　金：国家自然科学基金 (编号 :3 0 40 0 3 5 4);广东省自然科学基金 (编号 :0 43 0 0 5 0 2 )资助课题

摘　　要：目的　研究酵母mRNA在哺乳动物细胞中的翻译表达。方法　半乳糖诱导GFP基因在酿酒酵母细胞中表达,提取其mRNA ,RT -PCR鉴定,并以脂质体为介导,对血管内皮细胞HUVEC以及绿猴肾细胞COS - 7进行体外转染。结果　被转染HUVEC和COS - 7细胞分别出现绿色荧光,报告基因GFPmRNA在动物细胞中被识别、翻译。结论　提示酵母mRNA可能作为基因治疗物质应用于口服基因治疗的研究。Objective To study the possibility of translation and expression of mRNA from recombinant yeast in mammalian cells. Methods The expression of GFP gene in recombinant yeast was induced by galacose. The mRNA was extracted and identified by RT-PCR and was transfected with liposome into mammalian cell HUVEC and COS-7. Results Green fluorescence appeared in transfected cells and mRNA of GFP gene was translated and expressed in HUVEC and COS-7 cells. Conclusion mRNA of recombinant yeast may be used as novel gene therapy materials for oral gene therapy.

关 键 词：GFP 报告基因 酿酒酵母 MRNA 哺乳动物细胞 表达 

分 类 号：Q26[生物学—细胞生物学]