CCD微阵列生物芯片扫描仪的研制  

Research and Design of the CCD-based Microarray Scanner

作　　者：周强[1] 宗光华[1] 毕树生[1] 赵然[1] 

机构地区：[1]北京航空航天大学机器人研究所,北京100083

出　　处：《仪器仪表学报》2005年第2期164-167,176,共5页Chinese Journal of Scientific Instrument

基　　金：国家 8 63计划 (2 0 0 1AA42 2 190 )资助项目

摘　　要：报导了CCD微阵列生物芯片扫描仪的光学系统 ,给出了光学系统的参考标准构型 ,并依据此构型研制出多分辨率CCD生物芯片扫描仪。实验采用不同浓度系列Cy3NHSester的DMS0溶液样点与微池溶液测定CCD生物芯片扫描仪的检测性能。初步实验数据表明 ,此扫描仪光路合理 ,精度满足生物芯片检测要求。The optical system of the CCD-based microarray scanner has been analyzed. A novel CCD-based microarray scanner with multi-resolution has been developed. Microarray samples of Cy3 NHS ester spots on glass substrates and solutions in microwell with varying concentration are used to evaluate the performance of the scanner. Results of fluorescence intensity with varying dye concentration and with different exposure time are presented. Preliminary experiments have shown that the optical system of the scanner is optimal and the scanner has the key performance for microarray detection.

关 键 词：生物芯片扫描仪 CCD 微阵列 研制 光学系统 参考标准 多分辨率 检测性能 DMSO 检测要求 数据表 构型 实验 溶液 

分 类 号：TN29[电子电信—物理电子学] TB852.1[一般工业技术—摄影技术]