反相薄层色谱法拆分D,L-苯丙氨酸对映体  

SEPARATION OF ENANTIOMER OF PHENYLALANINE BY REVERSED-PHASE THIN LAYER CHROMATOGRAPHY

作　　者：方艳红[1] 王琼[1] 徐金瑞[1] 

机构地区：[1]华侨大学材料科学与工程学院,泉州362011

出　　处：《理化检验（化学分册）》2005年第4期241-242,244,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：研究了用β环糊精(βCD)作为流动相手性添加剂,在反相C18板上分离D,L苯丙氨酸对映体的薄层色谱法。流动相最佳组成配比为VβCD饱和溶液∶V甲酸∶V甲醇=9∶2∶1,最佳分离温度为50℃,D,L苯丙氨酸对映体相对比移值α为1.15。SEPARATION OF ENANTIOMER OF PHENYLALANINE BY REVERSED-PHASE THINLAYER CHROMATOGRAPHY$$$$ FANG Yanhong, WANG Qiong, XU Jinrui (College of Material Science and Engineering, Huaqiao University, Quanzhou 362011, China)Abstract: Racemes of phenylalanine can be separated on RPC18 aluminium sheets by thinlayer chromatography βcyclodextrinformic acidmethanol as developing system in which βcyclodextrin was used as chiral additive. The optimal composition of the mobile phase was βcyclodextrin solution∶formic acid∶methanol=9∶2∶1(V/V/V). The optimal separation temperature was 50 ℃. The values of relative Rf of Dphenylalanine and Lphenylalanine was 1.15. Keywords: Reversedphase thinlayer chromatography; βCyclodextrin; Phenylalanine; Enantiomer

关 键 词：反相薄层色谱法 Β-环糊精 苯丙氨酸 对映体 

分 类 号：O657.7[理学—分析化学]