复苏犬红细胞ATP酶活性及肿瘤坏死因子含量的变化及意义  

Changes and Meaning of Erythrocytic ATPase Activity and TNF Contents in Resuscitation Dogs

作　　者：王永进[1] 杨兴易[2] 赵良[2] 许永华[2] 李红江[2] 

机构地区：[1]河北省石家庄白求恩国际和平医院急诊科 [2]第二军医大学长征医院急救科,上海050082

出　　处：《齐齐哈尔医学院学报》2005年第2期126-127,共2页Journal of Qiqihar Medical University

摘　　要：目的　通过观测复苏犬红细胞 ATP酶活性及血肿瘤坏死因子(tumor necrosis factor,TNF)含量的变化,探讨复苏后血粘度增高及血流动力学紊乱的机制。方法　电击致犬室颤心跳呼吸骤停,15 min进行标准复苏,观察心脏停跳15 min、复苏后0.5 h、2 h、4 h时红细胞Na+-K+ATP酶、Ca2+ATP酶活性及血TNF含量。结果　心脏停跳及复苏后,Na+-K+ATP酶活性、Ca2+ ATP酶活性显著降低(P <0.05 vsP <0.05),血TNF含量明显升高(P <0.01)。相关分析显示:Ca2+ATP酶活性与TNF含量呈显著负相关 r =-0.5299,P <0.05)。结论　红细胞ATP酶活性降低可能导致了复苏后血液粘度升高,TNF含量升高与血流动力学不稳定有关,二者在复苏后的病理生理过程中可能起协同作用。Objective To investigate the mechanism of the increase of viscosity of blood and homodynamic disorder by way of observing the change of erythrocytic ATPase activity and serum TNF contents in resuscitation dogs. Methods Dogs were given electrical shock with ventricular fibrillation/cardiac arrest(CA) and normally resuscitated after 15 minutes. Na+-K+ATPase, Ca 2+ATPase activity of erythrocyte and serum TNF contents were measured at 15 minutes after CA, 0.5h, 2h, 4h post-resuscitation respectively. Results Erythrocytic Na+-K+ATPase,Ca 2+ATPase activity decreased significantly (P<0.05 vs. P<0.05)and serum TNF contents increased markedly(P<0.01)after 15min of CA and resuscitation. The number analyzed indicated there was significantly negative correlation(r= -0.5299,P<0.05)between Ca 2+ATPase activity and TNF contents. Conclusions Decrease of ATPase activity can result in the increase of viscosity of blood and TNF can participate in homodynamic instability, both of them can play an synergistic role during physiopathologic duration post-resuscitation.

关 键 词：红细胞ATP酶活性 肿瘤坏死因子 含量 血流动力学不稳定 Ca^2+ 血流动力学紊乱 factor 心跳呼吸骤停 血液粘度升高 病理生理过程 复苏后 TNF 心脏停跳 相关分析 活性降低 协同作用 血粘度 负相关 

分 类 号：R364.4[医药卫生—病理学] R285.5[医药卫生—基础医学]