动态比浊法测定葛根素葡萄糖注射液中细菌内毒素的含量  

Content Determination of Bacterial Endotoxin in Puerarin and Glucose Injection by Kinetic-turbidimetric Technique

作　　者：周学琴[1] 郭长杰[1] 

机构地区：[1]北京大学深圳医院药剂科,深圳市518036

出　　处：《中国药房》2005年第7期537-539,共3页China Pharmacy

摘　　要：目的:建立葛根素葡萄糖注射液中细菌内毒素的含量测定方法。方法:采用动态比浊法,将葛根素葡萄糖注射液作2倍稀释,通过预干扰试验和干扰试验,测定其中细菌内毒素的含量。结果:将样品作2倍稀释可有效消除对鲎试剂的干扰,测定其平均回收率在50 %～200 %范围内。结论:本方法与热原检查的家兔法和半定量凝胶法比较,具有检测灵敏度高、周期短、结果准确、重现性好等优点。OBJECTIVE:To establish a method for the contents determination of bacterial endotoxin in Puerarin and Glucose injection.METHODS:The kinetic-turbidimetric technique was adopted to dilute the puerarin glucose injection by2-fold,in which,the contents of bacterial endotoxin was determinated by preliminary interference test and interference test.RESULTS:Diluting the sample by2-fold could effectively eliminate its interference to tachypleus amebocyte lysate(TAL).The average recovery rate was at the range of50%～200%.CONCLUSLON:Compared with the pyrogen rabbit de-termination method and the half-quantity gel method,the kinetic-turbidimetic technique has many merits such as high de-termination sensitivity,short cycle,accurate result and better reproduction quality,etc.

关 键 词：葛根素葡萄糖注射液 动态比浊法 细菌内毒素 干扰试验 

分 类 号：R927.12[医药卫生—药学]