MPTP对小鼠空间学习记忆和脑内强啡肽免疫反应的影响  

EFFECTS OF MPTP ON THE ABILITY OF SPATIAL LEARNING MEMORY AND EXPRESSION OF DYNORPHIN IN C57BL/6 MICE

作　　者：殷盛明[1] 肖昭扬[2] 高溪[1] 王世伟[1] 于德钦[1] 唐一源[3] 张万琴[1] 

机构地区：[1]大连医科大学生理学教研室,大连116027 [2]大连医科大学第一附属医院麻醉科,大连116011 [3]大连理工大学神经信息研究所,大连116027

出　　处：《神经解剖学杂志》2005年第2期159-164,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(No. 60472017)资助项目

摘　　要：为了观察1 -甲基- 4 -苯基- 1, 2, 3, 6 四氢吡啶(MPTP)对C57BL/6小鼠空间学习记忆能力和脑内强啡肽(DYN)表达强度的影响,本研究给予小鼠皮下注射MPTP20mg/kg,连续8d,制备Parkinson病(PD)模型。与对照组相比,MPTP处理小鼠爬竿(P<0. 01)和游泳(P<0. 01)分数明显降低。Morris水迷宫实验结果显示:MPTP处理小鼠寻找平台潜伏期明显延长(P<0.01),在目标及对侧象限游泳时间所占百分比分别明显降低(P<0. 01)和增加(P<0. 01)。免疫细胞化学结果表明中脑黑质致密部的酪氨酸羟化酶(TH)免疫阳性神经元数目明显减少(P<0. 01)。同时在前额叶皮层(P<0. 01 )、背侧海马的CA1区(P<0. 05)和齿状回苔状纤维通路(P<0. 05)、背侧尾核(P<0. 01)的DYN免疫反应活性明显增强。本实验结果表明:用MPTP处理诱发的PD小鼠模型伴有空间学习和记忆能力的下降;并提示前额叶皮层、背侧海马的CA1区、齿状回苔状纤维通路和背侧尾核内DYN表达的增多可能与MPTP小鼠空间学习和记忆能力下降有关。To observe the effects of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)on the ability of spatial learning memory and expression of dynorphin in C57BL /6 mice, Parkinson's disease (PD) model was made by injecting MPTP (20 mg/kg) subcutaneously into mice for 8 days .The scores in the pole ( P <0.01) and swim test( P <0.01) was much lower than that of the control ones. Morris water maze showed the search latency of MPTP-treated mice was much longer than that of the control ( P <0.01). Compared with the control group, the percentage of time spending in the target quadrant was decreased ( P <0.01) and the quadrant opposite increased ( P <0.01) significantly. Immunocytochemistry showed large loss of tyrosine hydroxylase-immunoreactive (TH-IR) positive neurons in the pars conpacta of the substantia nigra ( P <0.01). Meanwhile, the immnnoreactivity of dynorphin (DYN-IR) positive neurons in prefrontal cortex( P <0.01), CA1 region( P <0.05), dendate gyrus granule cells-mossy fiber pathway ( P <0.05)of dorsal hippocampus and dorsal caudatum( P <0.01) increased significantly compared with that of the control. The results indicated that PD mice induced by MPTP accompanied with the deficits of the spatial learning and memory, and the increase of DYN-IR neurons in prefrontal cortex, CA1 region and dendate gyrus granule cells-mossy fiber pathway of dorsal hippocampus, dorsal caudatum may be related to the deficits of the spatial learning and memory in MPTP-treated mice.

关 键 词：MPTP 小鼠 空间学习记忆 强啡肽 免疫反应 1-甲基4-苯基-1 2 3 6-四氢吡啶 PARKINSON病 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R338.64[医药卫生—临床医学]