糖基化终极产物对乳鼠心肌细胞胞内游离钙瞬间变化的影响  

Transient cytosolic free calcium changes in cultured neonatal rat cardiac myocytes in response to advanced glycation end-product treatment

作　　者：邵亚辉[1] 许顶立[1] 曾平[1] 赖文岩[1] 任昊[2] 江荣炎[1] 

机构地区：[1]南方医科大学南方医院心血管内科,广东广州510515 [2]南方医科大学南方医院肾内科,广东广州510515

出　　处：《第一军医大学学报》2005年第3期274-276,280,共4页Journal of First Military Medical University

基　　金：国家重点基础研究发展规划(G2000056905);广东省自然科学基金研究团队项目(10717)~~

摘　　要：目的探讨糖基化终极产物(AGEs)刺激对体外培养乳鼠心肌细胞内游离钙的影响.方法应用荧光钙离子指示剂 Fluo-3/AM将体外培养3～5 d的乳鼠心肌细胞染色,激光共聚焦显微镜(LSCM)观察不同浓度AGEs(50、100、200μg/ml)对心肌细胞瞬时刺激后细胞内游离钙离子浓度的影响.结果与对照组相比,AGEs刺激可以迅速引起心肌细胞胞内游离钙浓度的升高,其恢复时程显著延长并呈剂量依赖性.结论AGEs可能通过改变心肌细胞内游离钙离子浓度对心肌细胞造成损伤.Objective To investigate the effects of advanced glycation end-products (AGEs) on transient cytosolic free calcium in neonatal rat cardiac myocytes (CMs) cultured in vitro. Methods CMs cultured for 3 to 5 days in vitro were incubated with Ca2+-sensitive fluorescent indicator Fluo-3AM with light screening at 37 ℃ with 5% CO2 for 60 min. Changes of the fluorescence signal of free calcium caused by AGEs were measured under laser scanning confocal microscope (LSCM). Results Compared with the control cells, AGEs caused an increase in the concentration of cytosolic free calcium in a dose-dependent manner. Conclusion AGEs may impair neonatal rat CMs by altering cytosolic free calcium concentration.

关 键 词：糖基化终极产物 心肌细胞 游离钙 细胞内 激光共聚焦显微镜 

分 类 号：R363[医药卫生—病理学]