IL-18抗体对急性免疫性肝坏死动物模型的影响  

The effect of IL-18 on the establishment of acute immunized hepatonecrosis animal model

作　　者：吴建成[1] 王泽荣[1] 万千红[1] 徐杰[1] 

机构地区：[1]苏州大学附属第一医院感染病科,215006

出　　处：《江苏医药》2005年第4期286-288,F003,共4页Jiangsu Medical Journal

摘　　要：目的探讨白细胞介素(IL) 18 抗体干预,了解IL 18 抗体对急性免疫性肝坏死动物模型的影响。方法取BALB/c 小鼠18 只,随机平分为三组A组(正常对照组);采用结核菌苗(BCG)加脂多糖(LPS)诱导法建立急性免疫性肝损伤模型B组(单纯病理组);抗体治疗组(C组)在模型形成前用IL 18抗体干预。实验结束取所有BALB/c小鼠肝组织,行病理学肝组织炎症活动度计分(HAI),血清IL 18由ELISA法检测,肝组织IL 18、肿瘤坏死因子α(INF α)、干扰素γ(IFN γ)由RT PCR检测。结果C组HAI 3 333±1 633,明显低于B组19 000±0 548(P<0 05);B组血清IL 18明显较C组增高(P<0 05);B组肝组织IL 18、IFN γ、INF αmRNA表达水平明显较C组增高(P<0 05)。结论IL 18、IFN γ、TNF α在急性免疫性肝坏死动物模型的形成中发挥重要作用,IL 18抗体可干预其形成。Objective To explore the effect of interleukin-18(IL-18) interference on the acute immunized hepatonecrosis animal model.Methods 18 BALB/c mice were divided into three groups randomly with 6 rats each.Group A served as a control.Acute immunized hepatonecrosis animal model was established with BCG+LPS.The rats in group C were treated with anti-IL-18 before establishment of the model.After experiment,hepatic tissue HAI was performed,and serum IL-18?TNF-α?IFN-γ were chected by RT-PCR.Results The HAI in group C was 3.333±1.633,which was sigifigantly lower than that in group B(19.000±0.548)(P<0.05).Serum IL-18 in group B was significantly higher than that in group C(P<0.05).The IL-18,TFN-γ,and TNF-α mRNA in the hepatic tissues in group B were markedly higher than those in group C(P<0.05).Conclusion The IL-18,TFN-γ and TNF-α play a important pole in establishing acute immunized hepatonecrosis animal model,and anti-IL-18 may interfere with the formation of acute immunized hepatonecrosis.

关 键 词：动物模型 肝坏死 抗体 急性 BALB/c小鼠 血清IL-18 免疫性肝损伤模型 脂多糖(LPS) 肝组织炎症活动度 RT-PCR检测 IFN-γ 肿瘤坏死因子α INF-Α mRNA表达 白细胞介素 正常对照组 EuSA法 TNF-α 结核菌苗 干扰素γ B组 

分 类 号：R575.302[医药卫生—消化系统] R392.11[医药卫生—内科学]