缺氧对培养人视网膜色素上皮细胞线粒体酶活性的影响  

Influence of hypoxia on mitochondria enzyme activity in cultured human retinal pigment epithelial cells

作　　者：朱洁[1] 王雨生[1] 张金山[2] 张鹏[1] 王海燕[1] 赵炜[1] 

机构地区：[1]第四军医大学西京医院眼科,陕西省西安市全军眼科研究所710032 [2]第四军医大学基础部组胚教研室

出　　处：《眼科新进展》2005年第2期105-108,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号 :30 371516 ) ;教育部留学回国人员科研启动基金资助 ( 2 0 0 4 ) ;陕西省自然科学基金资助 (编号 :2 0 0 4C2 4 6 ) ;第四军医大学科技创新工程资助 (编号 :CX0 2A0 2 1);第四军医大学西京医院科技创新基金资助 (编号 :XJCX0 4 ) ~

摘　　要：目的　研究缺氧对培养的人视网膜色素上皮(retinalpigmentepithelium ,RPE)细胞线粒体琥珀酸脱氢酶(succinatedehydrogenase,SDH)和细胞色素氧化酶(cytochromeoxi dase,COX)活性的影响,以及缺氧对RPE细胞增生的作用。方法　氯化钴(CoCl2 )法建立培养人RPE细胞缺氧模型。于缺氧后0 .5h、1h、2h、4h、6h、8h、16h和2 4h ,用组织化学法测定SDH和COX活性。于缺氧后第1天、2天、3天、4天和5天,用四甲基偶氮唑蓝[3(4,5dimethylthiazole 2 yl) 2 ,5diphenyltetrazoliumbromide,MTT]比色法检测RPE细胞增生。结果　RPE细胞SDH和COX活性随缺氧时间延长而逐渐降低,与对照组有显著性差异(P <0 .0 5 )。缺氧组RPE细胞增生能力增强(P <0 .0 5 )。结论　缺氧可使RPE细胞线粒体酶活性降低,并刺激RPE细胞增生。Objective To investigate the influence of hypoxia on the activities of mitochondrial succinate dehydrogenase and cytochrome oxidase in cultured human retinal pigment epithelial (RPE) cells and the effect of hypoxia on the cellular proliferation of RPE cells.Methods The hypoxia model was set up using Cobalt Chloride.After (hypoxia) 0.5 hour,1 hour,2 hours,4 hours,6 hours,8 hours,16 hours and 24 hours,the mitochondrial enzyme activity was determined with histochemistry technique.After hypoxia 1 day,2 days,3 days,4 days and 5 days,the proliferation of RPE cells was evaluated by [3 (4,5 dimethylthiazole 2yl) 2,5 diphenyl tetrazolium bromide,MTT] assay.Results The mitochondrial succinate dehydrogenase and cytochrome oxidase activity of RPE cells in the hypoxia group showed a significant suppression compared with the normal group(P<0.05).The cellular proliferation of RPE cells in the hypoxia group was increased(P<0.05).Conclusion Hypoxia can suppress the mitochondrial enzyme activity in cultured human RPE cells and stimulate the proliferation of human RPE cells.Similar study may help to answer the questions involved in the pathogenesis of hypoxic-ischemic chorioretinal diseases.

关 键 词：视网膜色素上皮 缺氧 琥珀酸脱氢酶 细胞色素氧化酶 细胞增生 

分 类 号：R774.1[医药卫生—眼科]