人雌激素受体cDNA在酵母细胞中的表达  

The expression of human estrogen receptor cDNA in yeast cell

作　　者：李湘鸣[1] 罗方妮[2] 陈春波[3] 陈秀云[1] 朱网娣[1] 

机构地区：[1]扬州大学医学院,江苏扬州225001 [2]扬州大学动物科学与技术学院,江苏扬州225009 [3]扬州大学兽医学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2005年第1期19-22,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：教育部留学回国人员科研启动基金资助项目(PK0407149)江苏省高校自然科学基金资助项目(03KJB610168)江苏省卫生厅医学科技发展基金(Z200328)扬州大学高级人才启动基金资助项目(2003-35)

摘　　要：根据人全长雌激素受体cDNA序列,用RT-PCR方法使乳腺癌细胞MCF-7株扩增人雌激素受体基因(hERcDNA),通过pGEM-T/hER载体克隆到酵母表达载体中,并转化到酿酒酵母细胞中,用Westernblot法和免疫组化法检测hERcDNA的表达。结果表明:hERcDNA在酵母细胞中成功表达,可用于建立环境雌激素的酵母细胞检测体系。The hER cDNA was amplified by RT-PCR method from breast cancer cell, MCF-7, according to the sequence of human full-length of estrogen receptor cDNA. The gene was cloned into the yeast expression vector via pGEM-T/hER vector, which was transformed into Saccharomyces cerevisae. And then Western blot and immunohis-tochemistry methods were used to assay the expression of hER cDNA in the yeast cell. The result showed that hER cDNA was expressed successfully in yeast cell,so as to construct the yeast system to evaluate environmental estrogenic hormone.

关 键 词：环境雌激素 酵母 雌激素受体 基因表达 

分 类 号：Q579.1[生物学—生物化学] Q786