细胞周期蛋白G2 mRNA在急性白血病患者中的表达及临床意义  

Expression of Cyclin G2 mRNA in Patients with Acute Leukemia and Its Clinical Significance

作　　者：贾晋松[1] 徐世荣[1] 马劼[1] 哈森[2] 郭晓楠[1] 王颖[1] 

机构地区：[1]河北医科大学第二医院血液内科 [2]内蒙古医学院第一附属医院血液内科,呼和浩特010050

出　　处：《中国实验血液学杂志》2005年第2期254-259,共6页Journal of Experimental Hematology

基　　金：河北省科委基金资助项目(02276104d-13)

摘　　要：为了探讨急性白血病(AL)患者中细胞周期蛋白G2(cyclin G2)的mRNA表达及其临床意义,采用逆转录 聚合酶链反应(RT-PCR)法检测74例急性白血病患者及10例正常人骨髓细胞中的cyclin G2、G1和P53的mRNA 表达,对cyclin G2的阳性扩增产物进行序列分析。结果表明:①AL患者中cyclin G2的阳性率及mRNA表达水平 (52.7％,0.552±0.498)明显低于正常人(100％,1.953±0.675)(P<0.01)。②在初治的AL患者中,cyclin G2 阳性表达患者的完全缓解(CR)率高于阴性者(分别为69.2％和40％;P<0.05)。③耐药组AL患者cyclin G2的 阳性率(43.6％)明显低于敏感组(68.6％)(P<0.05)。④对初治28例CR的AL患者进行随访14.3个月(11- 18.5个月),11例cyclin G2低表达患者中有10例复发(90.9％),而17例cyclin G2高表达患者中有7例复发 (41.2％),两者之间有显著性差异(P<0.05)。结论:CyclinG2是影响AL患者CR的因素,其高表达可能是AL患 者良好预后的指标之一。To evaluate the expression of cyclin G2 mRNA in patients with acute leukaemia (AL) and its clinical value, the expression of cyclin G2,G1 and P53 mRNA in the bone marrow from 74 AL patients and 10 normal individuals as control were detected with reverse transcription polymerase chain reaction (RT-PCR). The positive segment of cyclin G2 was analyzed by DNA sequencing. The results showed that (1) the positive rate and the expressing level of cyclin G2 in AL patients(52.7% , 0.552 ±0.498) were significantly lower than those in normal control (100% , 1.953 ±0.675) (P<0.01) ; (2)among new diagnosed AL patients, the complete remission(CR)rate (69.2% ) in the positive cyclin G2 patients was higher than that(40% ) in negative cyclin G2 patients (P < 0. 05); (3) the positive rate of cyclin G2 (43.6% ) in resistance group was significantly higher than that(68.6% )in sensitive group (P<0.01); (4) following-up for 14.3 month( 11 - 18. 5 month) in 28 AL patients with CR, there were 10 relapsed in 11 AL patients with low expression level of cyclin G2 (90.9% ); and 7 relapsed in 17 AL patients with high expression(41. 2% ), and there was significant difference (P < 0.05). In conclusion, the expression of cyclin G2 in AL patients was higher than that in normal control, the abnormal expression of cyclin G2 might be a prognostic marker of CR in AL patients .

关 键 词：细胞周期蛋白G2 细胞周期蛋白G1 急性白血病 基因表达 

分 类 号：R733.71[医药卫生—肿瘤]